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c-Jun (pThr91) antibody

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Anti c-Jun (pThr91) Antibody gallery image 1
Western blot analysis of extracts from HEK-293 cells untreated or treated with UV (for 15-30 minutes) using Rabbit anti c-Jun (pThr91) antibody

Rabbit anti c-Jun (pThr91)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2579
Datasheet
WB
SDS
50 µl loader

Rabbit anti c-Jun (pThr91) antibody recognizes proto-oncogene c-Jun, also known as V-jun avian sarcoma virus 17 oncogene homolog and p39, when phosphorylated at threonine 91.

c-Jun is a member of the Jun family of transcription factors and is a subunit of the AP-1 transcription factor. c-Jun dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.

Phosphorylation of c-Jun at threonines 91 and 93 is thought to result in a conformational change of c-Jun (Papavassiliou et al. 1995).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to c-Jun (pThr91) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 91 of human c-Jun

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P05412
Entrez Gene
JUN
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003702 RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005100 Rho GTPase activator activity
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007184 SMAD protein import into nucleus
GO:0008063 Toll signaling pathway
GO:0008134 transcription factor binding
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043392 negative regulation of DNA binding
GO:0043922 negative regulation by host of viral transcription
GO:0043923 positive regulation by host of viral transcription
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0060395 SMAD protein signal transduction
GO:0070412 R-SMAD binding
Regulatory
For research purposes only

Applications of c-Jun (pThr91) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Papavassiliou, A.G. et al. (1995) Intramolecular signal transduction in c-Jun.
    EMBO J. 14 (9): 2014-9.

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