Rabbit anti c-Jun (pSer73) antibody recognizes proto-oncogene c-Jun, also known as transcription factor AP-1, activator protein 1 (AP1), V-jun avian sarcoma virus 17 oncogene homolog and p39, when phosphorylated at serine 73. The c-Jun transcription factor is encoded by the c-jun oncogene and dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.
c-Jun becomes phosphorylated on Serine 63 and Serine 73 by MAPK8 (SAPK/JNK) and PLK3, which regulate c-Jun transcription. c-Jun has been implicated in early tumor development, invasion and metastasis and due to these findings has been identified as a target for cancer therapies.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 50% Glycerol
1% Bovine serum Albumin
- Synthetic phospho-peptide corresponding to residues surrounding Ser73 of human C-Jun
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
RNA polymerase II transcription factor activity
sequence-specific enhancer binding RNA polymerase II transcription factor activity
transcription coactivator activity
Rho GTPase activator activity
transforming growth factor beta receptor signaling pathway
SMAD protein import into nucleus
Toll signaling pathway
transcription factor binding
transcription activator activity
transcription repressor activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
negative regulation of DNA binding
negative regulation by host of viral transcription
positive regulation by host of viral transcription
innate immune response
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription factor activity
stress-activated MAPK cascade
SMAD protein signal transduction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of c-Jun (pSer73) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti c-Jun (pSer 73) recognizes a band of approximately 48 kDa in lysate from NIH3T3 cells treated with the MAP kinase inhibitor anisomycin
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Secondary Antibodies Available