Rabbit anti c-Jun (pSer63) antibody
recognizes proto-oncogene c-Jun, also known as V-jun avian sarcoma virus 17 oncogene homolog and p39, when phosphorylated at serine 63.
c-Jun is a member of the Jun family of transcription factors and is a subunit of the AP-1 transcription factor. c-Jun dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.
c-Jun is phosphorylated at serine 63 and serine 73 by MAPK8 (SAPK1/JNK1) and PLK3, resulting in c-Jun mediated transcription (Wang et al. 2007
, Vleugel et al. 2006
). c-Jun has been implicated in early tumor development, invasion and metastasis while c-Jun phosphorylated at serine 63 has been proposed to play a role in intestinal tumor progression (Madeo et al. 2010
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to c-Jun (pSer63) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 63 of human c-Jun
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
RNA polymerase II transcription factor activity
sequence-specific enhancer binding RNA polymerase II transcription factor activity
transcription coactivator activity
Rho GTPase activator activity
transforming growth factor beta receptor signaling pathway
SMAD protein import into nucleus
Toll signaling pathway
transcription factor binding
transcription activator activity
transcription repressor activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
negative regulation of DNA binding
negative regulation by host of viral transcription
positive regulation by host of viral transcription
innate immune response
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription factor activity
stress-activated MAPK cascade
SMAD protein signal transduction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of c-Jun (pSer63) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Wang, L. et al. (2007) Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells.
J Biol Chem. 282 (44): 32121-7.
Vleugel, M.M. et al. (2006) c-Jun activation is associated with proliferation and angiogenesis in invasive breast cancer.
Hum Pathol. 37 (6): 668-74.
Madeo, A. et al. (2010) c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells.
Oncogene. 29 (7): 978-91.
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