c-Jun (pSer63) antibody

Rabbit anti c-Jun (pSer63)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
c-Jun (pSer63)

Product Code Applications Pack Size List Price Your Price Qty
AHP2576
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl
List Price Your Price

Rabbit anti c-Jun (pSer63) antibody recognizes proto-oncogene c-Jun, also known as V-jun avian sarcoma virus 17 oncogene homolog and p39, when phosphorylated at serine 63.

c-Jun is a member of the Jun family of transcription factors and is a subunit of the AP-1 transcription factor. c-Jun dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.

c-Jun is phosphorylated at serine 63 and serine 73 by MAPK8 (SAPK1/JNK1) and PLK3, resulting in c-Jun mediated transcription (Wang et al. 2007, Vleugel et al. 2006). c-Jun has been implicated in early tumor development, invasion and metastasis while c-Jun phosphorylated at serine 63 has been proposed to play a role in intestinal tumor progression (Madeo et al. 2010).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to c-Jun (pSer63) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Proclin™300
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 63 of human c-Jun
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
ProClin™ is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Wang, L. et al. (2007) Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells.
    J Biol Chem. 282 (44): 32121-7.
  2. Vleugel, M.M. et al. (2006) c-Jun activation is associated with proliferation and angiogenesis in invasive breast cancer.
    Hum Pathol. 37 (6): 668-74.
  3. Madeo, A. et al. (2010) c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells.
    Oncogene. 29 (7): 978-91.

UniProt
P05412
Entrez Gene
JUN
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003702 RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005100 Rho GTPase activator activity
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007184 SMAD protein import into nucleus
GO:0008063 Toll signaling pathway
GO:0008134 transcription factor binding
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043392 negative regulation of DNA binding
GO:0043922 negative regulation by host of viral transcription
GO:0043923 positive regulation by host of viral transcription
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0060395 SMAD protein signal transduction
GO:0070412 R-SMAD binding

AHP2576

141538

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