c-Fos antibody
Rabbit anti c-Fos antibody recognizes c-Fos also known as G0/G1 switch regulatory protein 7 (G0S7). The c-Fos transcription factor is encoded by the c-Fos proto-oncogene and dimerizes with c-Jun, JDP and ATF family members to form the AP-1 transcription factor (Eferl and Wagner 2003).
c-Fos plays an essential role in mediating cellular signaling responses after cellular stress. The ability of the c-Fos protein to act as a transcription factor is regulated by phosphorylation on selected sites by the kinases p38, ERK, RSK1 and RSK2 (Lopez-Bergami et al. 2011). The protein accumulates in the nucleus, where it acts as a transcription factor upon formation of heterodimers with c-Jun. This binding prevents the nuclear export and subsequent proteosomal degradation of c-Fos (Halazonetiset al. 1988).
c-Fos plays an essential role in mediating cellular signaling responses after cellular stress. The ability of the c-Fos protein to act as a transcription factor is regulated by phosphorylation on selected sites by the kinases p38, ERK, RSK1 and RSK2 (Lopez-Bergami et al. 2011). The protein accumulates in the nucleus, where it acts as a transcription factor upon formation of heterodimers with c-Jun. This binding prevents the nuclear export and subsequent proteosomal degradation of c-Fos (Halazonetiset al. 1988).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antiserum to c-Fos was raised by repeated immunisation of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Peptide mapping to the amino terminus of human c-Fos
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P01100 Related reagents
- Entrez Gene
- FOS Related reagents
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0006306 DNA methylation
- GO:0006954 inflammatory response
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0008063 Toll signaling pathway
- GO:0010843 promoter binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0034614 cellular response to reactive oxygen species
- GO:0045087 innate immune response
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- GO:0060395 SMAD protein signal transduction
- GO:0070412 R-SMAD binding
- Regulatory
- For research purposes only
Applications of c-Fos antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti c-Fos recognizes a band of approximately 55 kDa when run under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
