C-ABL antibody
Rabbit anti C-ABL antibody recognizes tyrosine-protein kinase, also known as ABL1 and JTK7. It was first identified as an oncogene associated with chromosome translocations in human leukemias (Greuber at al. 2013). It regulates cell growth and survival, oxidative stress, DNA-damage responses and cell migration (Hantschel and Superti-Furga 2004). C-ABL plays role in the cellular response to growth factors (Plattner et al. 1999). It phosphorylates EGF R and regulates receptor endocytosis (Tanos and Pendergast 2006). Enhanced C-ABL expression and activation has been detected in solid tumors (Greuber et al. 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to C-ABL was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- A synthetic peptide corresponding to amino acids 533-545 of human C-ABL
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00519
- Entrez Gene
- ABL1
- GO Terms
- GO:0000115 regulation of transcription involved in S phase of mitotic cell cycle
- GO:0000287 magnesium ion binding
- GO:0003677 DNA binding
- GO:0030036 actin cytoskeleton organization
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0051019 mitogen-activated protein kinase binding
- GO:0005524 ATP binding
- GO:0005730 nucleolus
- GO:0005856 cytoskeleton
- GO:0006298 mismatch repair
- GO:0007411 axon guidance
- GO:0008022 protein C-terminus binding
- GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
- GO:0017124 SH3 domain binding
- GO:0018108 peptidyl-tyrosine phosphorylation
- GO:0030145 manganese ion binding
- GO:0031965 nuclear membrane
- GO:0042692 muscle cell differentiation
- GO:0048471 perinuclear region of cytoplasm
- GO:0051149 positive regulation of muscle cell differentiation
- GO:0051353 positive regulation of oxidoreductase activity
- GO:0070064 proline-rich region binding
- GO:0071901 negative regulation of protein serine/threonine kinase activity
- Regulatory
- For research purposes only
Applications of C-ABL antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/100 | 1/200 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti C-ABL antibody detects a band of approximately 139 kDa in HeLa cell lysate under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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