C-ABL antibody

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Rabbit anti C-ABL

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2718
Datasheet
P WB
MSDS
50 µl loader

Rabbit anti C-ABL antibody recognizes tyrosine-protein kinase, also known as ABL1 and JTK7. It was first identified as an oncogene associated with chromosome translocations in human leukemias (Greuber at al. 2013). It regulates cell growth and survival, oxidative stress, DNA-damage responses and cell migration (Hantschel and Superti-Furga 2004). C-ABL plays role in the cellular response to growth factors (Plattner et al. 1999). It phosphorylates EGF R and regulates receptor endocytosis (Tanos and Pendergast 2006). Enhanced C-ABL expression and activation has been detected in solid tumors (Greuber et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to C-ABL was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
A synthetic peptide corresponding to amino acids 533-545 of human C-ABL

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P00519
Entrez Gene
ABL1
GO Terms
GO:0000115 regulation of transcription involved in S phase of mitotic cell cycle
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0030036 actin cytoskeleton organization
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0051019 mitogen-activated protein kinase binding
GO:0005524 ATP binding
GO:0005730 nucleolus
GO:0005856 cytoskeleton
GO:0006298 mismatch repair
GO:0007411 axon guidance
GO:0008022 protein C-terminus binding
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0017124 SH3 domain binding
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030145 manganese ion binding
GO:0031965 nuclear membrane
GO:0042692 muscle cell differentiation
GO:0048471 perinuclear region of cytoplasm
GO:0051149 positive regulation of muscle cell differentiation
GO:0051353 positive regulation of oxidoreductase activity
GO:0070064 proline-rich region binding
GO:0071901 negative regulation of protein serine/threonine kinase activity
Regulatory
For research purposes only

Applications of C-ABL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti C-ABL antibody detects a band of approximately 139 kDa in HeLa cell lysate under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml