BRCC36 antibody

Rabbit anti BRCC36

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BRCC36

Product Code Applications Pack Size List Price Your Price Qty
AHP1484
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Human BRCC36 antibody recognizes human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7 kDa nuclear protein.

BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity.

BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation and BRCC36 is aberrantly expressed in the majority of breast tumors indicating a potential role in the pathogenesis of the disease. BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumors (Chen et al. 2004).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human BRCC36 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
An 18 amino acid peptide from near the amino terminus of human BRCC36.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1484 detects a band of approximately 36kDa in MCF7 cell lysates.

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Further Reading

  1. Dong, Y. et al. (2003) Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair.
    Mol Cell. 12 (5): 1087-99.
  2. Chen, X. et al. (2006) BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation.
    Cancer Res. 66:5039-46.

RRID
AB_2290662
UniProt
Q53YX5
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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