BRCC36 antibody
Rabbit anti Human BRCC36 antibody recognizes human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7 kDa nuclear protein.
BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity.
BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation and BRCC36 is aberrantly expressed in the majority of breast tumors indicating a potential role in the pathogenesis of the disease. BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumors (Chen et al. 2004).
BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity.
BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation and BRCC36 is aberrantly expressed in the majority of breast tumors indicating a potential role in the pathogenesis of the disease. BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumors (Chen et al. 2004).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human BRCC36 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- An 18 amino acid peptide from near the amino terminus of human BRCC36.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q53YX5
- Regulatory
- For research purposes only
Applications of BRCC36 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1484 detects a band of approximately 36kDa in MCF7 cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Dong, Y. et al. (2003) Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair.
Mol Cell. 12 (5): 1087-99. -
Chen, X. et al. (2006) BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation.
Cancer Res. 66:5039-46.
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