BRCA2 antibody

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Rabbit anti Human BRCA2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0300-0023 C P* 1 ml
Rabbit anti Human BRCA2 antibody recognizes human Breast Cancer Type 2 susceptibility protein (BRCA2), a tumour-suppressor protein reported to be involved in double-strand break repair, homologous recombination and S-phase checkpoint activation.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Horse Expected from Sequence
Cat Expected from Sequence
Chimpanzee Expected from Sequence
Rhesus Monkey Expected from Sequence
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 2587 - 2601 of the human BRCA2 precursor, conjugated to Keyhole Limpet Haemocyanin.

Peptide sequence: LIPSNDGKAGKEEFY
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P51587 Related reagents
Entrez Gene
BRCA2 Related reagents
GO Terms
GO:0000724 double-strand break repair via homologous recombination
GO:0002020 protease binding
GO:0003697 single-stranded DNA binding
GO:0005654 nucleoplasm
GO:0030141 stored secretory granule
GO:0005813 centrosome
GO:0006289 nucleotide-excision repair
GO:0007090 regulation of S phase of mitotic cell cycle
GO:0010484 H3 histone acetyltransferase activity
GO:0010485 H4 histone acetyltransferase activity
GO:0016563 transcription activator activity
GO:0033205 cell cycle cytokinesis
GO:0033593 BRCA2-MAGE-D1 complex
GO:0033600 negative regulation of mammary gland epithelial cell proliferation
GO:0043015 gamma-tubulin binding
GO:0043966 histone H3 acetylation
GO:0043967 histone H4 acetylation
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0051298 centrosome duplication
Regulatory
For research purposes only

Applications of BRCA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/25 1/100
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P