BMP-7 antibody

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Rabbit anti BMP-7

BMP-7 antibody recognizes bone morphogenetic protein 7, also known as osteogenic protein 1, or OP-1.

BMP-7 Antibody with Control Lysate

BMP-7 antibody recognizes bone morphogenetic protein 7, also known as osteogenic protein 1, or OP-1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00829 WB 100 µl
VPA00829KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human BMP-7 antibody recognizes the bone morphogenetic protein 7, also known as osteogenic protein 1 (OP-1).

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by BMP7 has a proposed role in early development and possible bone inductive activity (provided by RefSeq, Jul 2008).

Rabbit anti Human BMP-7 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti BMP-7 antibody recognizes a band of approximately 49 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide corresponding to a portion of BMP-7
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P18075 Related reagents
Entrez Gene
BMP7 Related reagents
GO Terms
GO:0001503 ossification
GO:0001657 ureteric bud development
GO:0001823 mesonephros development
GO:0001837 epithelial to mesenchymal transition
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030501 positive regulation of bone mineralization
GO:0030509 BMP signaling pathway
GO:0032582 negative regulation of gene-specific transcription
GO:0034504 protein localization to nucleus
GO:0042326 negative regulation of phosphorylation
GO:0043401 steroid hormone mediated signaling pathway
GO:0043407 negative regulation of MAP kinase activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0045839 negative regulation of mitosis
GO:0051216 cartilage development
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060395 SMAD protein signal transduction
GO:0072125 negative regulation of glomerular mesangial cell proliferation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of BMP-7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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