BMP-7 antibody
Rabbit anti BMP-7
BMP-7 antibody recognizes bone morphogenetic protein 7, also known as osteogenic protein 1, or OP-1.
BMP-7 Antibody with Control Lysate
BMP-7 antibody recognizes bone morphogenetic protein 7, also known as osteogenic protein 1, or OP-1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00829 | WB | 100 µl | ||||
| VPA00829KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human BMP-7 antibody recognizes the bone morphogenetic protein 7, also known as osteogenic protein 1 (OP-1).
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by BMP7 has a proposed role in early development and possible bone inductive activity (provided by RefSeq, Jul 2008).
Rabbit anti Human BMP-7 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by BMP7 has a proposed role in early development and possible bone inductive activity (provided by RefSeq, Jul 2008).
Rabbit anti Human BMP-7 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti BMP-7 antibody recognizes a band of approximately 49 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide corresponding to a portion of BMP-7
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P18075 Related reagents
- Entrez Gene
- BMP7 Related reagents
- GO Terms
- GO:0001503 ossification
- GO:0001657 ureteric bud development
- GO:0001823 mesonephros development
- GO:0001837 epithelial to mesenchymal transition
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0040007 growth
- GO:0008083 growth factor activity
- GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
- GO:0030501 positive regulation of bone mineralization
- GO:0030509 BMP signaling pathway
- GO:0032582 negative regulation of gene-specific transcription
- GO:0034504 protein localization to nucleus
- GO:0042326 negative regulation of phosphorylation
- GO:0043401 steroid hormone mediated signaling pathway
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045839 negative regulation of mitosis
- GO:0051216 cartilage development
- GO:0060389 pathway-restricted SMAD protein phosphorylation
- GO:0060395 SMAD protein signal transduction
- GO:0072125 negative regulation of glomerular mesangial cell proliferation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of BMP-7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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