BMP-7 antibody

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Rabbit anti Human BMP-7

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP961 E P* WB 50 µg
Rabbit anti Human BMP-7 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 7 (BMP-7) or Osteogenic protein 1 (OP-1). BMP-7 is a member of the transforming growth factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation, and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (3-4).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human BMP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human BMP-7.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P18075 Related reagents
Entrez Gene
BMP7 Related reagents
GO Terms
GO:0001503 ossification
GO:0001657 ureteric bud development
GO:0001823 mesonephros development
GO:0001837 epithelial to mesenchymal transition
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030501 positive regulation of bone mineralization
GO:0030509 BMP signaling pathway
GO:0032582 negative regulation of gene-specific transcription
GO:0034504 protein localization to nucleus
GO:0042326 negative regulation of phosphorylation
GO:0043401 steroid hormone mediated signaling pathway
GO:0043407 negative regulation of MAP kinase activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0045839 negative regulation of mitosis
GO:0051216 cartilage development
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060395 SMAD protein signal transduction
GO:0072125 negative regulation of glomerular mesangial cell proliferation
Regulatory
For research purposes only

Applications of BMP-7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for BMP-7 antibody

  1. Delpech, P.O. et al. (2014) Effects of warm ischaemia combined with cold preservation on the hypoxia-inducible factor 1α pathway in an experimental renal autotransplantation model.
    Br J Surg. 101 (13): 1739-50.

Further Reading

  1. Balemans, W. & Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
    Dev Biol. 250 (2): 231-50.
  2. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction.
    Biol Chem. 385 (8): 697-710.
  3. Wang, S.N. et al. (2001) Loss of tubular bone morphogenetic protein-7 in diabetic nephropathy.
    J Am Soc Nephrol. 12 (11): 2392-9.
  4. Godin, R.E. et al. (1998) Regulation of BMP7 expression during kidney development.
    Development. 125 (17): 3473-82.