BMP-7 antibody
Rabbit anti Human BMP-7 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 7 (BMP-7) or Osteogenic protein 1 (OP-1). BMP-7 is a member of the transforming growth factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation, and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.
BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.
Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (3-4).
BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.
Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (3-4).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human BMP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant human BMP-7.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P18075
- Entrez Gene
- BMP7
- GO Terms
- GO:0001503 ossification
- GO:0001657 ureteric bud development
- GO:0001823 mesonephros development
- GO:0001837 epithelial to mesenchymal transition
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0040007 growth
- GO:0008083 growth factor activity
- GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
- GO:0030501 positive regulation of bone mineralization
- GO:0030509 BMP signaling pathway
- GO:0032582 negative regulation of gene-specific transcription
- GO:0034504 protein localization to nucleus
- GO:0042326 negative regulation of phosphorylation
- GO:0043401 steroid hormone mediated signaling pathway
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045839 negative regulation of mitosis
- GO:0051216 cartilage development
- GO:0060389 pathway-restricted SMAD protein phosphorylation
- GO:0060395 SMAD protein signal transduction
- GO:0072125 negative regulation of glomerular mesangial cell proliferation
- Regulatory
- For research purposes only
Applications of BMP-7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5ug/ml | ||
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.1 | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for BMP-7 antibody
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Delpech, P.O. et al. (2014) Effects of warm ischaemia combined with cold preservation on the hypoxia-inducible factor 1α pathway in an experimental renal autotransplantation model.
Br J Surg. 101 (13): 1739-50.
Further Reading
-
Balemans, W. & Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
Dev Biol. 250 (2): 231-50. -
Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction.
Biol Chem. 385 (8): 697-710. -
Wang, S.N. et al. (2001) Loss of tubular bone morphogenetic protein-7 in diabetic nephropathy.
J Am Soc Nephrol. 12 (11): 2392-9. -
Godin, R.E. et al. (1998) Regulation of BMP7 expression during kidney development.
Development. 125 (17): 3473-82.
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