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BMP-7 antibody

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Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human BMP-7 (AHP961) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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Rabbit anti Human BMP-7

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BMP-7

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Product Code Applications Pack Size List Price Your Price Qty
AHP961
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 50 µg loader
List Price Your Price
loader

Rabbit anti Human BMP-7 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 7 (BMP-7) or Osteogenic protein 1 (OP-1). BMP-7 is a member of the transforming growth factor beta (TGF-B) superfamily and one of a number of osteogenic proteins shown to induce bone and cartilage formation, and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

BMP-7 is involved during ontogeny of the kidney with prominent expression in renal tubules. Experimental diabetic nephropathy indicates BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (Wang et al. 2001 & Godin et al. 1998).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human BMP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant human BMP-7.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for BMP-7 antibody

  1. Delpech, P.O. et al. (2014) Effects of warm ischaemia combined with cold preservation on the hypoxia-inducible factor 1α pathway in an experimental renal autotransplantation model.
    Br J Surg. 101 (13): 1739-50.

Further Reading

  1. Trousse, F. et al. (2001) Bmp4 mediates apoptotic cell death in the developing chick eye.
    J Neurosci. 21 (4): 1292-301.
  2. Balemans, W. & Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
    Dev Biol. 250 (2): 231-50.
  3. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction.
    Biol Chem. 385 (8): 697-710.
  4. Wang, S.N. et al. (2001) Loss of tubular bone morphogenetic protein-7 in diabetic nephropathy.
    J Am Soc Nephrol. 12 (11): 2392-9.
  5. Godin, R.E. et al. (1998) Regulation of BMP7 expression during kidney development.
    Development. 125 (17): 3473-82.

Synonyms
OP-1
RRID
AB_566393
UniProt
P18075
Entrez Gene
BMP7
GO Terms
GO:0001503 ossification
GO:0001657 ureteric bud development
GO:0001823 mesonephros development
GO:0001837 epithelial to mesenchymal transition
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030501 positive regulation of bone mineralization
GO:0030509 BMP signaling pathway
GO:0032582 negative regulation of gene-specific transcription
GO:0034504 protein localization to nucleus
GO:0042326 negative regulation of phosphorylation
GO:0043401 steroid hormone mediated signaling pathway
GO:0043407 negative regulation of MAP kinase activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0045839 negative regulation of mitosis
GO:0051216 cartilage development
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060395 SMAD protein signal transduction
GO:0072125 negative regulation of glomerular mesangial cell proliferation

AHP961

170210R

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