Rabbit anti Human BMP-7 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 7 (BMP-7) or Osteogenic protein 1 (OP-1). BMP-7 is a member of the transforming growth factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation, and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.
BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.
Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (3-4).
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human BMP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Recombinant human BMP-7.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
ureteric bud development
epithelial to mesenchymal transition
growth factor activity
positive regulation of pathway-restricted SMAD protein phosphorylation
positive regulation of bone mineralization
BMP signaling pathway
negative regulation of gene-specific transcription
protein localization to nucleus
negative regulation of phosphorylation
steroid hormone mediated signaling pathway
negative regulation of MAP kinase activity
positive regulation of osteoblast differentiation
negative regulation of mitosis
pathway-restricted SMAD protein phosphorylation
SMAD protein signal transduction
negative regulation of glomerular mesangial cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of BMP-7 antibody
|Immunohistology - Paraffin 1
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for BMP-7 antibody
Delpech, P.O. et al. (2014) Effects of warm ischaemia combined with cold preservation on the hypoxia-inducible factor 1α pathway in an experimental renal autotransplantation model.
Br J Surg. 101 (13): 1739-50.
Balemans, W. & Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
Dev Biol. 250 (2): 231-50.
Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction.
Biol Chem. 385 (8): 697-710.
Wang, S.N. et al. (2001) Loss of tubular bone morphogenetic protein-7 in diabetic nephropathy.
J Am Soc Nephrol. 12 (11): 2392-9.
Godin, R.E. et al. (1998) Regulation of BMP7 expression during kidney development.
Development. 125 (17): 3473-82.
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