BMP-7 antibody

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Rabbit anti Human BMP-7

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP961	E P* WB			50 µg

Rabbit anti Human BMP-7 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 7 (BMP-7) or Osteogenic protein 1 (OP-1). BMP-7 is a member of the transforming growth factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation, and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta ^(3-4).

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human BMP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Recombinant human BMP-7.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P18075 Related reagents
Entrez Gene: BMP7 Related reagents
GO Terms: GO:0001503 ossification; GO:0001657 ureteric bud development; GO:0001823 mesonephros development; GO:0001837 epithelial to mesenchymal transition; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0040007 growth; GO:0008083 growth factor activity; GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation; GO:0030501 positive regulation of bone mineralization; GO:0030509 BMP signaling pathway; GO:0032582 negative regulation of gene-specific transcription; GO:0034504 protein localization to nucleus; GO:0042326 negative regulation of phosphorylation; GO:0043401 steroid hormone mediated signaling pathway; GO:0043407 negative regulation of MAP kinase activity; GO:0045669 positive regulation of osteoblast differentiation; GO:0045839 negative regulation of mitosis; GO:0051216 cartilage development; GO:0060389 pathway-restricted SMAD protein phosphorylation; GO:0060395 SMAD protein signal transduction; GO:0072125 negative regulation of glomerular mesangial cell proliferation
Regulatory: For research purposes only

Applications of BMP-7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		0.5ug/ml
Immunohistology - Paraffin ¹
Western Blotting	0.1	0.2ug/ml

¹ This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: AHP961 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AHP961B as the detection antibody and PHP168 as the standard.
Western Blotting: AHP961 may be used in Western blotting with PHP168 as the positive control.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

Product Specific References

References for BMP-7 antibody

Delpech, P.O. et al. (2014) Effects of warm ischaemia combined with cold preservation on the hypoxia-inducible factor 1α pathway in an experimental renal autotransplantation model.
Br J Surg. 101 (13): 1739-50.