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BMP-4 antibody

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Western Blot Validated
Anti BMP-4 Antibody (PrecisionAb™ Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with BMP-4 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to BMP-4 (molecular weight 47 kDa)

Rabbit anti BMP-4

Rabbit anti human BMP-4 antibody recognizes bone morphogenetic protein 4, also known as BMP4 or bone morphogenetic protein 2B (BMP-2B). This antibody has been extensively validated for western blotting using whole cell lysates.

BMP-4 Antibody with Control Lysate

Rabbit anti human BMP-4 antibody recognizes bone morphogenetic protein 4, also known as BMP4 or bone morphogenetic protein 2B (BMP-2B). This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00302 WB 100 µl
VPA00302KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human BMP-4 antibody recognizes bone morphogenetic protein 4 (BMP-4) also known as bone morphogenetic protein 2B (BMP-2B).

BMP-4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo at an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of the BMP-4 gene sequence has been described and three variants reported, all encoding an identical protein (provided by RefSeq, Jul 2008).

Rabbit anti Human BMP-4 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti BMP-4 detects a band of approximately 47 kDa in A549 cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the C-terminal region of human BMP-4
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P12644 Related reagents
Entrez Gene
BMP4 Related reagents
GO Terms
GO:0000186 activation of MAPKK activity
GO:0001649 osteoblast differentiation
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0001823 mesonephros development
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002043 blood vessel endothelial cell proliferation involved in sprouting angiogenesis
GO:0002320 lymphoid progenitor cell differentiation
GO:0003197 endocardial cushion development
GO:0003279 cardiac septum development
GO:0003337 mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0005125 cytokine activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0009791 post-embryonic development
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010595 positive regulation of endothelial cell migration
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030501 positive regulation of bone mineralization
GO:0032967 positive regulation of collagen biosynthetic process
GO:0033088 negative regulation of immature T cell proliferation in thymus
GO:0034504 protein localization to nucleus
GO:0042056 chemoattractant activity
GO:0042326 negative regulation of phosphorylation
GO:0043065 positive regulation of apoptosis
GO:0043401 steroid hormone mediated signaling pathway
GO:0043407 negative regulation of MAP kinase activity
GO:0045662 negative regulation of myoblast differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045839 negative regulation of mitosis
GO:0045843 negative regulation of striated muscle tissue development
GO:0048286 lung alveolus development
GO:0048392 intermediate mesodermal cell differentiation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0051216 cartilage development
GO:0060391 positive regulation of SMAD protein import into nucleus
GO:0060395 SMAD protein signal transduction
GO:0060433 bronchus development
GO:0060502 epithelial cell proliferation involved in lung morphogenesis
GO:0060503 bud dilation involved in lung branching
GO:0061036 positive regulation of cartilage development
GO:0061155 pulmonary artery endothelial tube morphogenesis
GO:0070244 negative regulation of thymocyte apoptosis
GO:0070700 BMP receptor binding
GO:0071893 BMP signaling pathway involved in nephric duct formation
GO:0072097 negative regulation of branch elongation involved in ureteric bud branching by BMP signaling pathway
GO:0072101 specification of ureteric bud anterior/posterior symmetry by BMP signaling pathway
GO:0072125 negative regulation of glomerular mesangial cell proliferation
GO:0090184 positive regulation of kidney development
GO:0090191 negative regulation of branching involved in ureteric bud morphogenesis
GO:0090194 negative regulation of glomerulus development
GO:2000005 negative regulation of metanephric S-shaped body morphogenesis
GO:2000007 negative regulation of metanephric comma-shaped body morphogenesis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of BMP-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
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