BMP-2 antibody

100% Secure


Rabbit anti Human BMP-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1728 E P* 50 µg
Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2, BMP-2 or BMP-2a), a 44.7 kDa secreted protein belonging to the TGF-beta superfamily.

BMP2 is one of a number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human BMP2 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to amino acids 86-102 of human CBMP2 – conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12643 Related reagents
Entrez Gene
BMP2 Related reagents
GO Terms
GO:0001649 osteoblast differentiation
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003130 BMP signaling pathway involved in heart induction
GO:0005125 cytokine activity
GO:0005160 transforming growth factor beta receptor binding
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0010922 positive regulation of phosphatase activity
GO:0016362 activin receptor activity, type II
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019211 phosphatase activator activity
GO:0030501 positive regulation of bone mineralization
GO:0035051 cardiac cell differentiation
GO:0035630 bone mineralization involved in bone maturation
GO:0043065 positive regulation of apoptosis
GO:0045669 positive regulation of osteoblast differentiation
GO:0045786 negative regulation of cell cycle
GO:0046332 SMAD binding
GO:0046982 protein heterodimerization activity
GO:0051216 cartilage development
GO:0060317 cardiac epithelial to mesenchymal transition
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060395 SMAD protein signal transduction
GO:0061036 positive regulation of cartilage development
GO:0070700 BMP receptor binding
Regulatory
For research purposes only

Applications of BMP-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat 1/1000
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P