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BMI-1 antibody

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Anti BMI-1 Antibody thumbnail image 1 Anti BMI-1 Antibody thumbnail image 2
Anti BMI-1 Antibody gallery image 1
Western blot analysis of whole cell lysate from K562 human erythromyeloblastoid cells probed with Rabbit anti Human BMI-1 (AHP1189) at 0.5(A), 1(B) and 2(C) ug/ml.
Anti BMI-1 Antibody gallery image 2
Immunocytochemical staining of K562 cells with Rabbit anti Human BMI-1 (AHP1189).

Rabbit anti BMI-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1189
Datasheet
C WB
SDS
0.1 mg loader

Rabbit anti BMI-1 antibody recognizes 37 kDa polycomb complex protein BMI-1, involved in segment specification, cell growth and maintenance, transcriptional regulation and chromatin modification. BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells, making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf.

BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.

The Notch ligand DLL4 (delta-like protein 4) enhances T cell proliferation, by directly influencing BMI-1 transcription.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human BMI-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
15 amino acid peptide sequence near the centre of human BMI-1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P35226
Entrez Gene
BMI1
GO Terms
GO:0000151 ubiquitin ligase complex
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0006350 transcription
GO:0007379 segment specification
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0030097 hemopoiesis
GO:0045449 regulation of transcription
GO:0048146 positive regulation of fibroblast proliferation
GO:0051443 positive regulation of ubiquitin-protein ligase activity
GO:0071535 RING-like zinc finger domain binding
Regulatory
For research purposes only

Applications of BMI-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 0.5 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Raaphorst, F. et al. (2003) Self-renewal of hematopoietic and leukemic stem cells: a central role for the Polycomb-group gene Bmi-1.
    Trends Immunol. 10:522-4.
  2. Alkema, M.J. et al. (1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1.
    Hum Mol Genet. 2 (10): 1597-603.
  3. Lessard, J. & Sauvageau, G. (2003) Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.
    Nature. 423 (6937): 255-60.

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