BMI-1 antibody
Rabbit anti BMI-1 antibody recognizes 37 kDa polycomb complex protein BMI-1, involved in segment specification, cell growth and maintenance, transcriptional regulation and chromatin modification. BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells, making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf.
BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.
The Notch ligand DLL4 (delta-like protein 4) enhances T cell proliferation, by directly influencing BMI-1 transcription.
BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.
The Notch ligand DLL4 (delta-like protein 4) enhances T cell proliferation, by directly influencing BMI-1 transcription.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human BMI-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- 15 amino acid peptide sequence near the centre of human BMI-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35226
- Entrez Gene
- BMI1
- GO Terms
- GO:0000151 ubiquitin ligase complex
- GO:0005737 cytoplasm
- GO:0005730 nucleolus
- GO:0006350 transcription
- GO:0007379 segment specification
- GO:0008270 zinc ion binding
- GO:0016568 chromatin modification
- GO:0030097 hemopoiesis
- GO:0045449 regulation of transcription
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0051443 positive regulation of ubiquitin-protein ligase activity
- GO:0071535 RING-like zinc finger domain binding
- Regulatory
- For research purposes only
Applications of BMI-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting | 0.5 | 1.0ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Raaphorst, F. et al. (2003) Self-renewal of hematopoietic and leukemic stem cells: a central role for the Polycomb-group gene Bmi-1.
Trends Immunol. 10:522-4. -
Alkema, M.J. et al. (1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1.
Hum Mol Genet. 2 (10): 1597-603. -
Lessard, J. & Sauvageau, G. (2003) Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.
Nature. 423 (6937): 255-60.
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