BIM antibody

Product Code Applications Pack Size List Price Your Price Qty
AHP933
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Human Bim antibody recognizes human Bcl-2 interacting mediator of cell death (Bim), also known as Bod.

Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway. Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w.

Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex. Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human Bim were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 22 to 40 of human Bim.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP933 detects a band of approximately 22kDa in K562 and A549 whole cell lysates.

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References for BIM antibody

  1. (2012) The Human Protein Atlas
    BCL2L11
  2. Shin, S.H. et al. (2015) Differential Protein Expression in Congenital and Acquired Cholesteatomas.
    PLoS One. 10 (9): e0137011.
  3. Seo, G.Y. et al. (2022) Epithelial HVEM maintains intraepithelial T cell survival and contributes to host protection.
    Sci Immunol. 7 (73): eabm6931.

Immunohistology - Paraffin

Synonyms
Bod
RRID
AB_566386
UniProt
O43521
Entrez Gene
BCL2L11
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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