BIM antibody

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Goat anti Human BIM (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2074 E WB 0.1 mg
Goat anti Human BIM antibody recognizes an epitope within the C-terminal (CT) region of the AD, ACD and ABCD isoforms of human BIM, otherwise known as Bcl-2 interacting mediator of cell death.

BIM is an apoptotic activator protein and member of the Bcl-2 family, which forms heterodimers with several anti-apoptotic proteins including BCL2, BHRF1, and MCL1, but not with the pro-apoptotic proteins BAD, BAX or BAK. BIM can be induced by nerve growth factor (NGF) and also by forkhead transcription factor FKHR-L1, implicating BIM in both neuronal and lymphocyte apoptosis.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human BIM (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-FNAYYARRLEK from the C-Terminal region of BIM (NP_619528; NP_996886; NP_619529).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O43521 Related reagents
Entrez Gene
BCL2L11 Related reagents
Regulatory
For research purposes only

Applications of BIM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 0.5 2.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2074 detects a band of approximately 23kDa in K562 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB