Find out more about A.I.den - our SearchBot

BID antibody

ISO 9001 Quality Assurance Manufacturing | Print
Anti Human BID (C-Terminal) Antibody thumbnail image 1
Anti Human BID (C-Terminal) Antibody gallery image 1
Western blotting analysis of A431 lysate (35μg protein in RIPA buffer) using Goat anti Human BID antibody (AHP1073) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Filter by Application:

WB

Goat anti Human BID (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BID
Region
(C-TERMINAL)

Quick Links:

Antibody Quality and Performance Guarantee Antibody Quality and Performance GuaranteeAntibody Quality and
Performance Guarantee
Unrivaled technical support Unrivalled technical supportUnrivaled
Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
AHP1073
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human BID antibody detects an epitope within the C-terminus of BID, a death agonist that heterodimerizes with other members of the Bcl-2 family of cell death regulators; including the agonist BAX or antagonist BCL2.

BID contains a BH3 domain, required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Protease-cleaved BID translocates to mitochondria where it results in cytochrome c release, leading to caspase activation and cell death. This constitutes the activation of the mitochondria death pathway (the intrinsic pathway) by the death stimuli.

BID has been shown to promote cell cycle progression into S phase, whilst BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. Deletion of BID inhibits carcinogenesis in the liver, and promotes tumorigenesis in the myeloid cells.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to BID (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-TYVRSLARNGMD from the C-terminus of BID.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Western Blotting 0.1 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1073 detects bands of approximately 26kDa and 24kDa in A431 cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for BID antibody

  1. Yin, X.M. (2006) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death.
    Gene. 369: 7-19.

RRID
AB_808344
UniProt
P55957
Entrez Gene
BID
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0005829 cytosol
GO:0005123 death receptor binding
GO:0005624 membrane fraction
GO:0005741 mitochondrial outer membrane
GO:0008625 induction of apoptosis via death domain receptors
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0051402 neuron apoptosis
View more products with BID specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products