BETA-1 Adrenergic Receptor antibody
Rabbit anti BETA-1 Adrenergic Receptor
Beta-1 adrenergic receptor antibody recognizes the beta-1 adrenergic receptor, also known as RHR, ADRB1, or BETA1AR .
BETA-1 Adrenergic Receptor Antibody with Control Lysate
Beta-1 adrenergic receptor antibody recognizes the beta-1 adrenergic receptor, also known as RHR, ADRB1, or BETA1AR .
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00859 | WB | 100 µl | ||||
| VPA00859KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human beta-1 adrenergic receptor antibody recognizes the beta-1 adrenergic receptor, also known as RHR, ADRB1 and BETA1AR .
The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in ADRB1 have been shown to affect the resting heart rate and can be involved in heart failure (provided by RefSeq, Jul 2008).
Rabbit anti Human beta-1 adrenergic receptor antibody detects bands of 50-55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in ADRB1 have been shown to affect the resting heart rate and can be involved in heart failure (provided by RefSeq, Jul 2008).
Rabbit anti Human beta-1 adrenergic receptor antibody detects bands of 50-55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti beta-1 adrenergic receptor antibody recognizes a band of approximately 50-55 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on Protein A
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the C-terminal region of human beta-1 adrenergic receptor
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P08588 Related reagents
- Entrez Gene
- ADRB1 Related reagents
- GO Terms
- GO:0004940 beta1-adrenergic receptor activity
- GO:0005887 integral to plasma membrane
- GO:0030819 positive regulation of cAMP biosynthetic process
- GO:0031694 alpha-2A adrenergic receptor binding
- GO:0046982 protein heterodimerization activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of BETA-1 Adrenergic Receptor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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