Bcl-6 antibody

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Rabbit anti Bcl-6

Rabbit anti human Bcl-6 antibody recognizes the B-cell lymphoma 6 protein, also known as B-cell lymphoma 5 protein (Bcl-5). This antibody has been extensively validated for western blotting using whole cell lysates.

Bcl-6 Antibody with Control Lysate

Rabbit anti human Bcl-6 antibody recognizes the B-cell lymphoma 6 protein, also known as B-cell lymphoma 5 protein (Bcl-5). This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00255 WB 100 µl
VPA00255KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human Bcl-6 antibody recognizes Bcl-6, also known as B-cell lymphoma 5 protein, B-cell lymphoma 6 protein, B-cell lymphoma 6 protein transcript, cys-his2 zinc finger transcription factor, lymphoma-associated zinc finger gene on chromosome 3, protein LAZ-3, zinc finger and BTB domain-containing protein 27, zinc finger protein 51 and zinc finger transcription factor BCL6S.

The protein encoded by the Bcl-6 gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcriptional corepressors. Bcl-6 is found to be frequently translocated and hyper-mutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for Bcl-6 (provided by RefSeq, Jul 2008).

Rabbit anti Human Bcl-6 protein antibody detects a band of 98 kDa. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 364-395 of human Bcl-6

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P41182 Related reagents
Entrez Gene
BCL6 Related reagents
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0002634 regulation of germinal center formation
GO:0002903 negative regulation of B cell apoptosis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0006974 response to DNA damage stimulus
GO:0008270 zinc ion binding
GO:0016564 transcription repressor activity
GO:0030308 negative regulation of cell growth
GO:0043065 positive regulation of apoptosis
GO:0043565 sequence-specific DNA binding
GO:0045749 negative regulation of S phase of mitotic cell cycle
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Bcl-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti Bcl-6 detects a band of approximately 98 kDa in Ramos cell lysate
Western Blotting
Anti Bcl-6 detects a band of approximately 98 kDa in Ramos cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB