Rabbit anti Human BAP31 antibody recognizes the human chaperone protein, B-cell receptor-associated protein 31, also known as BAP31. BAP31 is a 246 amino acid ~28 kDa conserved multi pass type III integral transmembrane protein of the endoplasmic reticulum, containing two caspase cleavage sites within its cytosolic domain.

BAP31 appears to be a multi-functional ER-associated transport protein which was originally identified through its association with membrane IgD in B-cells (Adachi et al. 1996). Several proteins have since been shown to associate with BAP31, including the Beta2 integrin receptor CD11b/CD18 (Zen et al. 2004), non-muscle gamma-actin and myosin B heavy chain (Ducret et al. 2003). BAP31 has become a focus area for apoptosis studies due to its association with Bcl-2 and cleavage by initiator caspases including caspase 1 and 8 generating a membrane-integrated p20 fragment which is itself a potent inducer of apoptosis when expressed ectopically (Breckenridge et al. 2003).

Rabbit anti Human BAP31 antibody detects BAP31 as a band of approximately 24 kDa in a wide range of human cell line lysates.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000