BAP31 antibody
Rabbit anti BAP31
BAP31 antibody recognizes B cell receptor-associated protein 31, also known as BCR-associated protein 31, 6C6-AG tumor-associated antigen, protein CDM or p28. The antibody has been extensively validated for western blotting using whole cell lysates.
BAP31 Antibody with Control Lysate
BAP31 antibody recognizes B cell receptor-associated protein 31, also known as BCR-associated protein 31, 6C6-AG tumor-associated antigen, protein CDM or p28. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00088 | WB | 100 µl | ||||
| VPA00088KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human BAP31 antibody recognizes the human chaperone protein, B-cell receptor-associated protein 31, also known as BAP31. BAP31 is a 246 amino acid ~28 kDa conserved multi pass type III integral transmembrane protein of the endoplasmic reticulum, containing two caspase cleavage sites within its cytosolic domain.
BAP31 appears to be a multi-functional ER-associated transport protein which was originally identified through its association with membrane IgD in B-cells (Adachi et al. 1996). Several proteins have since been shown to associate with BAP31, including the Beta2 integrin receptor CD11b/CD18 (Zen et al. 2004), non-muscle gamma-actin and myosin B heavy chain (Ducret et al. 2003). BAP31 has become a focus area for apoptosis studies due to its association with Bcl-2 and cleavage by initiator caspases including caspase 1 and 8 generating a membrane-integrated p20 fragment which is itself a potent inducer of apoptosis when expressed ectopically (Breckenridge et al. 2003).
Rabbit anti Human BAP31 antibody detects BAP31 as a band of approximately 24 kDa in a wide range of human cell line lysates.
BAP31 appears to be a multi-functional ER-associated transport protein which was originally identified through its association with membrane IgD in B-cells (Adachi et al. 1996). Several proteins have since been shown to associate with BAP31, including the Beta2 integrin receptor CD11b/CD18 (Zen et al. 2004), non-muscle gamma-actin and myosin B heavy chain (Ducret et al. 2003). BAP31 has become a focus area for apoptosis studies due to its association with Bcl-2 and cleavage by initiator caspases including caspase 1 and 8 generating a membrane-integrated p20 fragment which is itself a potent inducer of apoptosis when expressed ectopically (Breckenridge et al. 2003).
Rabbit anti Human BAP31 antibody detects BAP31 as a band of approximately 24 kDa in a wide range of human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti BAP31 detects a band of approximately 24 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- 17 amino acid peptide located near human BAP31 C-terminus.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P51572 Related reagents
- Entrez Gene
- BCAP31 Related reagents
- GO Terms
- GO:0016192 vesicle-mediated transport
- GO:0005102 receptor binding
- GO:0005829 cytosol
- GO:0005887 integral to plasma membrane
- GO:0005789 endoplasmic reticulum membrane
- GO:0006886 intracellular protein transport
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0006955 immune response
- GO:0033116 ER-Golgi intermediate compartment membrane
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of BAP31 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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