BAP31 antibody

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Rabbit anti BAP31

BAP31 antibody recognizes B cell receptor-associated protein 31, also known as BCR-associated protein 31, 6C6-AG tumor-associated antigen, protein CDM or p28. The antibody has been extensively validated for western blotting using whole cell lysates.

BAP31 Antibody with Control Lysate

BAP31 antibody recognizes B cell receptor-associated protein 31, also known as BCR-associated protein 31, 6C6-AG tumor-associated antigen, protein CDM or p28. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00088 WB 100 µl
VPA00088KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human BAP31 antibody recognizes the human chaperone protein, B-cell receptor-associated protein 31, also known as BAP31. BAP31 is a 246 amino acid ~28 kDa conserved multi pass type III integral transmembrane protein of the endoplasmic reticulum, containing two caspase cleavage sites within its cytosolic domain.

BAP31 appears to be a multi-functional ER-associated transport protein which was originally identified through its association with membrane IgD in B-cells (Adachi et al. 1996). Several proteins have since been shown to associate with BAP31, including the Beta2 integrin receptor CD11b/CD18 (Zen et al. 2004), non-muscle gamma-actin and myosin B heavy chain (Ducret et al. 2003). BAP31 has become a focus area for apoptosis studies due to its association with Bcl-2 and cleavage by initiator caspases including caspase 1 and 8 generating a membrane-integrated p20 fragment which is itself a potent inducer of apoptosis when expressed ectopically (Breckenridge et al. 2003).

Rabbit anti Human BAP31 antibody detects BAP31 as a band of approximately 24 kDa in a wide range of human cell line lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti BAP31 detects a band of approximately 24 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
17 amino acid peptide located near human BAP31 C-terminus.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P51572 Related reagents
Entrez Gene
BCAP31 Related reagents
GO Terms
GO:0016192 vesicle-mediated transport
GO:0005102 receptor binding
GO:0005829 cytosol
GO:0005887 integral to plasma membrane
GO:0005789 endoplasmic reticulum membrane
GO:0006886 intracellular protein transport
GO:0006921 cellular component disassembly involved in apoptosis
GO:0006955 immune response
GO:0033116 ER-Golgi intermediate compartment membrane
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of BAP31 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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