BAK antibody
Rabbit anti BAK (N-Terminal) antibody recognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to 13 amino acids near the amino terminus of human BAK.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q16611
- O08734
- Entrez Gene
- Bak1
- BAK1
- GO Terms
- GO:0001782 B cell homeostasis
- GO:0001783 B cell apoptosis
- GO:0001836 release of cytochrome c from mitochondria
- GO:0001974 blood vessel remodeling
- GO:0002262 myeloid cell homeostasis
- GO:0002352 B cell negative selection
- GO:0051726 regulation of cell cycle
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0046872 metal ion binding
- GO:0008053 mitochondrial fusion
- GO:0008283 cell proliferation
- GO:0008635 activation of caspase activity by cytochrome c
- GO:0009620 response to fungus
- GO:0010046 response to mycotoxin
- GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
- GO:0010332 response to gamma radiation
- GO:0010524 positive regulation of calcium ion transport into cytosol
- GO:0046930 pore complex
- GO:0031966 mitochondrial membrane
- GO:0032471 reduction of endoplasmic reticulum calcium ion concentration
- GO:0033137 negative regulation of peptidyl-serine phosphorylation
- GO:0035108 limb morphogenesis
- GO:0043065 positive regulation of apoptosis
- GO:0046902 regulation of mitochondrial membrane permeability
- GO:0048597 post-embryonic camera-type eye morphogenesis
- GO:0051881 regulation of mitochondrial membrane potential
- GO:0060068 vagina development
- GO:0070059 apoptosis in response to endoplasmic reticulum stress
- GO:0005741 mitochondrial outer membrane
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0042802 identical protein binding
- GO:0043496 regulation of protein homodimerization activity
- GO:0043497 regulation of protein heterodimerization activity
- GO:0046982 protein heterodimerization activity
- Regulatory
- For research purposes only
Applications of BAK antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 2ug/ml | ||
| Western Blotting | 1 | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1782 detects a band of approximately 28kDa in L1210 cell lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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