Rabbit anti BAK (N-Terminal) antibody recognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- A peptide corresponding to 13 amino acids near the amino terminus of human BAK.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
B cell homeostasis
B cell apoptosis
release of cytochrome c from mitochondria
blood vessel remodeling
myeloid cell homeostasis
B cell negative selection
regulation of cell cycle
metal ion binding
activation of caspase activity by cytochrome c
response to fungus
response to mycotoxin
establishment or maintenance of transmembrane electrochemical gradient
response to gamma radiation
positive regulation of calcium ion transport into cytosol
reduction of endoplasmic reticulum calcium ion concentration
negative regulation of peptidyl-serine phosphorylation
positive regulation of apoptosis
regulation of mitochondrial membrane permeability
post-embryonic camera-type eye morphogenesis
regulation of mitochondrial membrane potential
apoptosis in response to endoplasmic reticulum stress
mitochondrial outer membrane
induction of apoptosis by intracellular signals
activation of pro-apoptotic gene products
identical protein binding
regulation of protein homodimerization activity
regulation of protein heterodimerization activity
protein heterodimerization activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of BAK antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1782 detects a band of approximately 28kDa in L1210 cell lysate
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Secondary Antibodies Available
Useful Reagents Available