BAK antibody

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Rabbit anti BAK (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1782 C WB 0.1 mg
Rabbit anti BAK (N-Terminal) antibody recognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to 13 amino acids near the amino terminus of human BAK.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q16611 Related reagents
O08734 Related reagents
Entrez Gene
Bak1 Related reagents
BAK1 Related reagents
GO Terms
GO:0001782 B cell homeostasis
GO:0001783 B cell apoptosis
GO:0001836 release of cytochrome c from mitochondria
GO:0001974 blood vessel remodeling
GO:0002262 myeloid cell homeostasis
GO:0002352 B cell negative selection
GO:0051726 regulation of cell cycle
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0008053 mitochondrial fusion
GO:0008283 cell proliferation
GO:0008635 activation of caspase activity by cytochrome c
GO:0009620 response to fungus
GO:0010046 response to mycotoxin
GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
GO:0010332 response to gamma radiation
GO:0010524 positive regulation of calcium ion transport into cytosol
GO:0046930 pore complex
GO:0031966 mitochondrial membrane
GO:0032471 reduction of endoplasmic reticulum calcium ion concentration
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0035108 limb morphogenesis
GO:0043065 positive regulation of apoptosis
GO:0046902 regulation of mitochondrial membrane permeability
GO:0048597 post-embryonic camera-type eye morphogenesis
GO:0051881 regulation of mitochondrial membrane potential
GO:0060068 vagina development
GO:0070059 apoptosis in response to endoplasmic reticulum stress
GO:0005741 mitochondrial outer membrane
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0043496 regulation of protein homodimerization activity
GO:0043497 regulation of protein heterodimerization activity
GO:0046982 protein heterodimerization activity
Regulatory
For research purposes only

Applications of BAK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 2ug/ml
Western Blotting 1 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1782 detects a band of approximately 28kDa in L1210 cell lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
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