BAG3 antibody
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WB ResetGoat anti BAG3
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- BAG3
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| Goat anti Human BAG3 antibody recognizes the BAG family molecular chaperone regulator 3 (BAG3), also known as Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis and docking protein CAIR-1. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. BAG3 contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner (provided by RefSeq, Jul 2008). Goat anti Human BAG3 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti BAG3 detects a band of approximately 85 kDa in MCF7 cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C-SSMTDTPGNPAAP, from the C terminus of the protein sequence.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Donkey anti Sheep/Goat IgG:HRP | ||||||
- UniProt
- O95817
- Entrez Gene
- BAG3
- GO Terms
- GO:0006457 protein folding
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0006916 anti-apoptosis
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