BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. BAG3 contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner (provided by RefSeq, Jul 2008).
Goat anti Human BAG3 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti BAG3 detects a band of approximately 85 kDa in MCF7 cell lysates.
- Product Form
- Purified IgG - liquid
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Peptide with the sequence C-SSMTDTPGNPAAP, from the C terminus of the protein sequence.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- Entrez Gene
- GO Terms
- GO:0006457 protein folding
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0006916 anti-apoptosis
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of BAG3 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||C E P WB||1 ml|