BAG3 antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti BAG3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human BAG3 antibody (**VPA00114**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to BAG3 (molecular weight 85 kDa).

Filter by Application:

Goat anti BAG3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: BAG3

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00114 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Goat anti Human BAG3 antibody recognizes the BAG family molecular chaperone regulator 3 (BAG3), also known as Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis and docking protein CAIR-1.

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. BAG3 contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner (provided by RefSeq, Jul 2008).

Goat anti Human BAG3 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti BAG3 detects a band of approximately 85 kDa in MCF7 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C-SSMTDTPGNPAAP, from the C terminus of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.