Rabbit anti Human BAG1 antibody recognizes the N-terminal (NT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70 kDa heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival.
The BAG1 gene encodes three different isoforms; a predominantly cytosolic form of ~36 kDa (BAG1S), a ~46 kDa cytosolic and nuclear form (BAG1M), and a predominantly nuclear form of ~50 kDa (BAG1L).
BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to BAG1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 14 amino acid peptide near the amino terminus of human BAG1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of BAG1 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1171 detects a band of approximately 35kDa in PC-3 cell lysate.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Götz, R. et al. (2005) Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells.
Nat Neurosci. 8 (9): 1169-78.
Liman, J. et al. (2005) Interaction of BAG1 and Hsp70 mediates neuroprotectivity and increases chaperone activity.
Mol Cell Biol. 25 (9): 3715-25.
Krajewska, M. et al. (2006) Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers.
Prostate. 66 (8): 801-10.
Clemo, N.K. et al. (2005) The role of the retinoblastoma protein (Rb) in the nuclear localization of BAG-1: implications for colorectal tumour cell survival.
Biochem Soc Trans. 33 (Pt 4): 676-8.
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