BAG1 antibody

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Rabbit anti BAG1 (N-Terminal)

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Rabbit anti Human BAG1 antibody recognizes the N-terminal (NT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70 kDa heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival.

The BAG1 gene encodes three different isoforms; a predominantly cytosolic form of ~36 kDa (BAG1S), a ~46 kDa cytosolic and nuclear form (BAG1M), and a predominantly nuclear form of ~50 kDa (BAG1L).

BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to BAG1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
14 amino acid peptide near the amino terminus of human BAG1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
For research purposes only

Applications of BAG1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1171 detects a band of approximately 35kDa in PC-3 cell lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Götz, R. et al. (2005) Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells.
    Nat Neurosci. 8 (9): 1169-78.
  2. Liman, J. et al. (2005) Interaction of BAG1 and Hsp70 mediates neuroprotectivity and increases chaperone activity.
    Mol Cell Biol. 25 (9): 3715-25.
  3. Krajewska, M. et al. (2006) Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers.
    Prostate. 66 (8): 801-10.
  4. Clemo, N.K. et al. (2005) The role of the retinoblastoma protein (Rb) in the nuclear localization of BAG-1: implications for colorectal tumour cell survival.
    Biochem Soc Trans. 33 (Pt 4): 676-8.

Fluorescent Spectraviewer

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