BACH1 antibody

Rabbit anti Human BACH1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BACH1

Product Code Applications Pack Size List Price Your Price Qty
AHP1426
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 50 µg loader
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loader

Rabbit anti Human BACH1 antibody recognizes the human Transcription regulator protein BACH1, also known as BTB and CNC homolog 1 or HA2303. BACH1 is a 736 amino acid, ~82 kDa nuclear transcription regulatory protein belonging to the bZIP family, containing a single BTP (POZ) domain and a single bZIP domain.

BACH1 is found in a wide range of tissues and acts as a transcriptional regulator. It plays an important role in the coordination of transcription in association with small Maf transcription factors. BACH-1 is inactivated by direct binding to intracellular heme, allowing induction of genes such as heme oxygenase-1 (Hada et al. 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Chimpanzee
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human BACH1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein .
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10000 1/44000
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 1/500 1/2000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human skeletal muscle

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RRID
AB_2061837
UniProt
O14867
Entrez Gene
BACH1
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0043565 sequence-specific DNA binding
GO:0046983 protein dimerization activity

AHP1426

154639

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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