BACH1 antibody
Rabbit anti Human BACH1 antibody recognizes the human Transcription regulator protein BACH1, also known as BTB and CNC homolog 1 or HA2303. BACH1 is a 736 amino acid, ~82 kDa nuclear transcription regulatory protein belonging to the bZIP family, containing a single BTP (POZ) domain and a single bZIP domain.
BACH1 is found in a wide range of tissues and acts as a transcriptional regulator. It plays an important role in the coordination of transcription in association with small Maf transcription factors. BACH-1 is inactivated by direct binding to intracellular heme, allowing induction of genes such as heme oxygenase-1 (Hada et al. 2014).
BACH1 is found in a wide range of tissues and acts as a transcriptional regulator. It plays an important role in the coordination of transcription in association with small Maf transcription factors. BACH-1 is inactivated by direct binding to intracellular heme, allowing induction of genes such as heme oxygenase-1 (Hada et al. 2014).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Chimpanzee Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human BACH1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein .
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O14867
- Entrez Gene
- BACH1
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0043565 sequence-specific DNA binding
- GO:0046983 protein dimerization activity
- Regulatory
- For research purposes only
Applications of BACH1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/10000 | 1/44000 | |
| Immunohistology - Paraffin 1 | 5ug/ml | ||
| Western Blotting | 1/500 | 1/2000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human skeletal muscle
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Fluorescent Spectraviewer
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- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
