BACE2 antibody

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Rabbit anti Human BACE2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP607 WB 0.1 mg
Rabbit anti Human BACE2 antibody recognizes human Beta-secretase 2, also known as BACE2, Aspartic-like protease 56 kDa, Aspartyl protease 1, Beta-site amyloid precursor protein cleaving enzyme 2, Down region aspartic protease, Memapsin-1, Membrane-associated aspartic protease 1 or Theta-secretase. BACE2 is a 456 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein bearing a 20 amino acid N-terminal signal peptide and an adjacent 42 amino acid pro-peptide region. Rabbit anti Human BACE2 antibody recognizes an epitope within the N-terminal region of BACE2, a homologue of the beta-site APP cleaving enzyme (BACE). BACE2 has been reported to cleave the beta-amyloid precursor protein (APP) at the beta-site, producing amyloid-beta protein (Yan et al.1999). Accumulation of amyloid beta plaques in the cerebral cortex play an important role in the pathogenesis of Alzheimer's disease, particularly in individuals bearing the Flemish mutation (Farzan et al. 2000).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human BACE2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 44-59 of human BACE2.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y5Z0 Related reagents
Entrez Gene
BACE2 Related reagents
GO Terms
GO:0004190 aspartic-type endopeptidase activity
GO:0016021 integral to membrane
GO:0005783 endoplasmic reticulum
GO:0005768 endosome
GO:0005794 Golgi apparatus
GO:0006509 membrane protein ectodomain proteolysis
GO:0009986 cell surface
GO:0016486 peptide hormone processing
GO:0042985 negative regulation of amyloid precursor protein biosynthetic process
Regulatory
For research purposes only

Applications of BACE2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for BACE2 antibody

  1. Koryakina A et al. (2009) Regulation of secretases by all-trans-retinoic acid.
    FEBS J. 276 (9): 2645-55.

Further Reading

  1. Farzan, M. et al. (2000) BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein.
    Proc. Natl. Acad. Sci. 97:9712-7.