Aurora-B Kinase antibody

Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3.
AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Expected from Sequence Bovine Expected from Sequence Mouse Expected from Sequence Chimpanzee Expected from Sequence Dog Expected from Sequence Pig Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human Aurora-B kinase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase.
- Approx. Protein Concentrations
- IgG concentration 0.2mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q96GD4
- Entrez Gene
- AURKB
- GO Terms
- GO:0005515 protein binding
- GO:0000236 mitotic prometaphase
- GO:0000780 condensed nuclear chromosome, centromeric region
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005819 spindle
- GO:0006468 protein phosphorylation
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0032133 chromosome passenger complex
- GO:0034501 protein localization to kinetochore
- Regulatory
- For research purposes only
Applications of Aurora-B Kinase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/2000 | 1/10000 | |
Western Blotting | 1/250 | 1/1000 |
- Western Blotting
- AHP1261 detects a band of approximately 39kDa in HeLa cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Vader, G. et al. (2006) The chromosomal passenger complex: guiding Aurora-B through mitosis.
J Cell Biol. 173 (6): 833-7. -
Keen, N. & Taylor, S. (2004) Aurora-kinase inhibitors as anticancer agents.
Nat Rev Cancer. 4 (12): 927-36.
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