ATR antibody

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Rabbit anti ATR (aa2381-2644)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP384 WB 0.1 ml
Rabbit anti peptide E antibody recognizes the ATR (Ataxia telangiectasia and rad 3 related) protein, a ~300 kD protein kinase reported to have a role in recognising and responding to DNA strand interruptions during meiosis.

Rabbit anti peptide E antibody cross-reacts with the related DNA-Pkcs.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Hamster
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human ATR peptide were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human ATR peptide, amino acids 2381-2644.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q13535 Related reagents
Entrez Gene
ATR Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000077 DNA damage checkpoint
GO:0003677 DNA binding
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006281 DNA repair
GO:0007049 cell cycle
GO:0007275 multicellular organismal development
GO:0008156 negative regulation of DNA replication
GO:0016605 PML body
GO:0032405 MutLalpha complex binding
GO:0032407 MutSalpha complex binding
GO:0034644 cellular response to UV
GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator
GO:0071480 cellular response to gamma radiation
GO:0090399 replicative senescence
Regulatory
For research purposes only

Applications of ATR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Western Blotting

Product Specific References

References for ATR antibody

  1. Keegan, K.S. et al. (1996) The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.
    Genes and Development 10: 2423-2437.
  2. McNees, C.J. et al. (2010) ATR suppresses telomere fragility and recombination but is dispensable for elongation of short telomeres by telomerase.
    J. Cell Biol. 188: 639-52
  3. Yan, T. et al. (2004) CHK1 and CHK2 are differentially involved in mismatch repair-mediated 6-thioguanine-induced cell cycle checkpoint responses.
    Mol Cancer Ther. 3: 1147-57.
  4. Cuadrado, M. et al. (2006) ATM regulates ATR chromatin loading in response to DNA double-strand breaks.
    J Exp Med. 203: 297-303.
  5. Zinkel, S.S. et al. (2005) A role for proapoptotic BID in the DNA-damage response.
    Cell. 122: 579-91.
  6. Murga, M. et al. (2009) A mouse model of ATR-Seckel shows embryonic replicative stress and accelerated aging.
    Nat Genet. 41: 891-8.
  7. Lewis, K.A. et al. (2005) Heterozygous ATR Mutations in Mismatch Repair–Deficient Cancer Cells Have Functional Significance
    Cancer Res. 65: 7091-5.
  8. Pontano, L.L. et al. (2008) Genotoxic stress-induced cyclin D1 phosphorylation and proteolysis are required for genomic stability.
    Mol Cell Biol. 28: 7245-58.
  9. Brown, E.J. and Baltimore, D. (2003) Essential and dispensable roles of ATR in cell cycle arrest and genome maintenance.
    Genes Dev. 17: 615-28.
  10. Park, B.J. et al. (2006) AIMP3 haploinsufficiency disrupts oncogene-induced p53 activation and genomic stability.
    Cancer Res. 66: 6913-8.
  11. López-Contreras, A.J. et al. (2012) An extra allele of Chk1 limits oncogene-induced replicative stress and promotes transformation.
    J Exp Med. 209 (3): 455-61.