Rabbit anti peptide E antibody recognizes the ATR (Ataxia telangiectasia and rad 3 related) protein, a ~300 kD protein kinase reported to have a role in recognising and responding to DNA strand interruptions during meiosis.
Rabbit anti peptide E antibody cross-reacts with the related DNA-Pkcs.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human ATR peptide were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- Human ATR peptide, amino acids 2381-2644.
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
DNA damage checkpoint
protein serine/threonine kinase activity
multicellular organismal development
negative regulation of DNA replication
MutLalpha complex binding
MutSalpha complex binding
cellular response to UV
positive regulation of DNA damage response, signal transduction by p53 class mediator
cellular response to gamma radiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ATR antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for ATR antibody
Keegan, K.S. et al. (1996) The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.
Genes and Development 10: 2423-2437.
McNees, C.J. et al. (2010) ATR suppresses telomere fragility and recombination but is dispensable for elongation of short telomeres by telomerase.
J. Cell Biol. 188: 639-52
Yan, T. et al. (2004) CHK1 and CHK2 are differentially involved in mismatch repair-mediated 6-thioguanine-induced cell cycle checkpoint responses.
Mol Cancer Ther. 3: 1147-57.
Cuadrado, M. et al. (2006) ATM regulates ATR chromatin loading in response to DNA double-strand breaks.
J Exp Med. 203: 297-303.
Zinkel, S.S. et al. (2005) A role for proapoptotic BID in the DNA-damage response.
Cell. 122: 579-91.
Murga, M. et al. (2009) A mouse model of ATR-Seckel shows embryonic replicative stress and accelerated aging.
Nat Genet. 41: 891-8.
Lewis, K.A. et al. (2005) Heterozygous ATR Mutations in Mismatch Repair–Deficient Cancer Cells Have Functional Significance
Cancer Res. 65: 7091-5.
Pontano, L.L. et al. (2008) Genotoxic stress-induced cyclin D1 phosphorylation and proteolysis are required for genomic stability.
Mol Cell Biol. 28: 7245-58.
Brown, E.J. and Baltimore, D. (2003) Essential and dispensable roles of ATR in cell cycle arrest and genome maintenance.
Genes Dev. 17: 615-28.
Park, B.J. et al. (2006) AIMP3 haploinsufficiency disrupts oncogene-induced p53 activation and genomic stability.
Cancer Res. 66: 6913-8.
López-Contreras, A.J. et al. (2012) An extra allele of Chk1 limits oncogene-induced replicative stress and promotes transformation.
J Exp Med. 209 (3): 455-61.
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