ATP5D antibody

Rabbit anti ATP5D

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
ATP5D

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VPA00673
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Rabbit anti Human ATP5D antibody recognizes ATP synthase subunit delta, mitochondrial, also known as ATP synthase subunit delta mitochondrial, F-ATPase delta subunit, mitochondrial ATP synthase complex delta-subunit precusor, or mitochondrial ATP synthase, delta subunit.

ATP5D gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5D gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified (provided by RefSeq, Jul 2008).

Rabbit anti Human ATP5D antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti ATP5D detects a band of approximately 17 kDa in MOLT-4 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human ATP5D
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P30049
Entrez Gene
ATP5D
GO Terms
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0000275 mitochondrial proton-transporting ATP synthase complex, catalytic core F(1)
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005759 mitochondrial matrix
GO:0022904 respiratory electron transport chain
GO:0006119 oxidative phosphorylation
GO:0042776 mitochondrial ATP synthesis coupled proton transport
GO:0046688 response to copper ion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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