ATP5A1 antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti ATP5A1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ATP5A1 antibody followed by detection with Donkey anti Sheep/Goat IgG (Fc):HRP (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to ATP5A1 (molecular weight 55 kDa).

Filter by Application:

Goat anti ATP5A1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ATP5A1

Quick Links:

Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00550 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Goat anti Human ATP5A1 antibody recognizes ATP5A1, also known as ATP synthase subunit alpha mitochondrial, non-cardiac muscle-like 2, ATP sythase (F1-ATPase) alpha subunit, epididymis secretory sperm binding protein Li 123m or mitochondrial ATP synthetase, oligomycin-resistant.

The ATP5A1 gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5A1 gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of ATP5A1 are located on chromosomes 9, 2, and 16 (provided by RefSeq, Mar 2012).

Goat anti Human ATP5A1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti ATP5A1 detects a band of approximately 55 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin.
Immunogen: Peptide sequence C-RVHGLRNVQAEE
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P25705
Entrez Gene: ATP5A1
GO Terms: GO:0005886 plasma membrane; GO:0046961 proton-transporting ATPase activity, rotational mechanism; GO:0001937 negative regulation of endothelial cell proliferation; GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism; GO:0005524 ATP binding; GO:0005759 mitochondrial matrix; GO:0022904 respiratory electron transport chain; GO:0006629 lipid metabolic process; GO:0009790 embryo development; View More GO Terms; GO:0042288 MHC class I protein binding; GO:0042776 mitochondrial ATP synthesis coupled proton transport; GO:0043499 eukaryotic cell surface binding