ATP5A1 antibody

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Goat anti ATP5A1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00550
Datasheet
WB
MSDS
100 µl

Goat anti Human ATP5A1 antibody recognizes ATP5A1, also known as ATP synthase subunit alpha mitochondrial, non-cardiac muscle-like 2, ATP sythase (F1-ATPase) alpha subunit, epididymis secretory sperm binding protein Li 123m or mitochondrial ATP synthetase, oligomycin-resistant.

The ATP5A1 gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5A1 gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of ATP5A1 are located on chromosomes 9, 2, and 16 (provided by RefSeq, Mar 2012).

Goat anti Human ATP5A1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATP5A1 detects a band of approximately 55 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence C-RVHGLRNVQAEE

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P25705
Entrez Gene
ATP5A1
GO Terms
GO:0005886 plasma membrane
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0001937 negative regulation of endothelial cell proliferation
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005524 ATP binding
GO:0005759 mitochondrial matrix
GO:0022904 respiratory electron transport chain
GO:0006629 lipid metabolic process
GO:0009790 embryo development
GO:0042288 MHC class I protein binding
GO:0042776 mitochondrial ATP synthesis coupled proton transport
GO:0043499 eukaryotic cell surface binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of ATP5A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader