ATP1B1 antibody

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Rabbit anti ATP1B1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00519
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human ATP1B1 antibody recognizes ATP1B1, also known as beta 1-subunit of Na(+),K(+)-ATPase, Na, K-ATPase beta-1 polypeptide or adenosinetriphosphatase.

The protein encoded by ATP1B1 gene belongs to the family of Na+/K+ and H+/K+ ATPase beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. ATP1B1 gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known (provided by RefSeq, Mar 2010).

Rabbit anti Human ATP1B1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATP1B1 detects a band of approximately 50 kDa in mouse brain tissue.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human ATP1B1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P05026
Entrez Gene
ATP1B1
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005391 sodium:potassium-exchanging ATPase activity
GO:0005890 sodium:potassium-exchanging ATPase complex
GO:0006754 ATP biosynthetic process
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of ATP1B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml