ATP1B1 antibody

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Anti ATP1B1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ATP1B1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to ATP1B1 (molecular weight 50 kDa).

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Rabbit anti ATP1B1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ATP1B1

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VPA00519 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human ATP1B1 antibody recognizes ATP1B1, also known as beta 1-subunit of Na(+),K(+)-ATPase, Na, K-ATPase beta-1 polypeptide or adenosinetriphosphatase.

The protein encoded by ATP1B1 gene belongs to the family of Na+/K+ and H+/K+ ATPase beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. ATP1B1 gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known (provided by RefSeq, Mar 2010).

Rabbit anti Human ATP1B1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.

Target Species: Human
Western Blotting: Anti ATP1B1 detects a band of approximately 50 kDa in mouse brain tissue.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the N terminal region of human ATP1B1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P05026
Entrez Gene: ATP1B1
GO Terms: GO:0005515 protein binding; GO:0007596 blood coagulation; GO:0005391 sodium:potassium-exchanging ATPase activity; GO:0005890 sodium:potassium-exchanging ATPase complex; GO:0006754 ATP biosynthetic process; GO:0050900 leukocyte migration