Rabbit anti ATM (pSer1981) antibody
recognizes serine-protein kinase ATM, also known as ataxia telangiectasia mutated, when phosphorylated at serine 1981. ATM is a serine/threonine protein kinase and a member of the PI3K/P14K-kinase family. ATM is a cell cycle checkpoint kinase that exists as an inactive dimer. In response to DNA damage, autophosphorylation of ATM at serine 1981 occurs, resulting in the dissociation of the dimer and initiating ATM kinase activity (Bakkenist and Kastan 2003
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ATM (pSer1981) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 1981 of human ATM
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
double-strand break repair via homologous recombination
pre-B cell allelic exclusion
DNA-dependent protein kinase activity
DNA damage induced protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
mitotic cell cycle spindle assembly checkpoint
reciprocal meiotic recombination
identical protein binding
cytoplasmic membrane-bounded vesicle
negative regulation of B cell proliferation
G2/M transition DNA damage checkpoint
protein complex binding
positive regulation of DNA damage response, signal transduction by p53 class mediator
protein dimerization activity
protein N-terminus binding
histone mRNA catabolic process
cellular response to gamma radiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ATM (pSer1981) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Bakkenist, C.J. & Kastan, M.B. (2003) DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
Nature. 421 (6922): 499-506.