ATM antibody

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Rabbit anti ATM (aa1-308)

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.1 ml loader

Rabbit anti ATM antibody recognizes the human ATM protein, a ~350 kDa polypeptide that is the product of the ATM gene. The ATM gene is defective in Ataxia telangiectasia. ATM protein is expressed within the nucleus of all normal cells, and is thought to be important in detection of DNA damage.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to ATM were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Recombinant human ATM fragment (amino acids 1-308).

Storage Information

Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.

More Information

Q13315 Related reagents
Entrez Gene
ATM Related reagents
GO Terms
GO:0000724 double-strand break repair via homologous recombination
GO:0002331 pre-B cell allelic exclusion
GO:0003677 DNA binding
GO:0004677 DNA-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006975 DNA damage induced protein phosphorylation
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007050 cell cycle arrest
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0007131 reciprocal meiotic recombination
GO:0042802 identical protein binding
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0030889 negative regulation of B cell proliferation
GO:0031572 G2/M transition DNA damage checkpoint
GO:0032403 protein complex binding
GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator
GO:0046777 protein autophosphorylation
GO:0046983 protein dimerization activity
GO:0047485 protein N-terminus binding
GO:0071044 histone mRNA catabolic process
GO:0071480 cellular response to gamma radiation
GO:0090399 replicative senescence
For research purposes only

Applications of ATM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1/100 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Western Blotting

Product Specific References

References for ATM antibody

  1. Lakin, N.D. et al. (1996) Analysis of the ATM protein in wild-type and ataxia telangiectasia cells.
    Oncogene. 13 (12): 2707-16.
  2. Jones, G.G. et al. (2004) The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes.
    Oncogene. 23 (10): 1911-21.
  3. Neal, J.A. et al. (2011) Inhibition of homologous recombination by DNA-dependent protein kinase requires kinase activity, is titratable, and is modulated by autophosphorylation.
    Mol Cell Biol. 31 (8): 1719-33.
  4. Shrivastav, M. et al. (2009) DNA-PKcs and ATM co-regulate DNA double-strand break repair.
    DNA Repair (Amst). 8: 920-9.
  5. Pettitt, A.R. et al. (2001) p53 dysfunction in B-cell chronic lymphocytic leukemia: inactivation of ATM as an alternative to TP53 mutation.
    Blood. 98 (3): 814-22.
  6. Henriksson, G. et al. (2004) Enhanced DNA-dependent protein kinase activity in Sjögren's syndrome B cells.
    Rheumatology (Oxford). 43: 1109-15.