Rabbit anti ATM antibody recognizes the human ATM protein, a ~350 kDa polypeptide that is the product of the ATM gene. The ATM gene is defective in Ataxia telangiectasia. ATM protein is expressed within the nucleus of all normal cells, and is thought to be important in detection of DNA damage.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to ATM were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- Recombinant human ATM fragment (amino acids 1-308).
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
double-strand break repair via homologous recombination
pre-B cell allelic exclusion
DNA-dependent protein kinase activity
DNA damage induced protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
mitotic cell cycle spindle assembly checkpoint
reciprocal meiotic recombination
identical protein binding
cytoplasmic membrane-bounded vesicle
negative regulation of B cell proliferation
G2/M transition DNA damage checkpoint
protein complex binding
positive regulation of DNA damage response, signal transduction by p53 class mediator
protein dimerization activity
protein N-terminus binding
histone mRNA catabolic process
cellular response to gamma radiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ATM antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for ATM antibody
Lakin, N.D. et al. (1996) Analysis of the ATM protein in wild-type and ataxia telangiectasia cells.
Oncogene. 13 (12): 2707-16.
Jones, G.G. et al. (2004) The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes.
Oncogene. 23 (10): 1911-21.
Neal, J.A. et al. (2011) Inhibition of homologous recombination by DNA-dependent protein kinase requires kinase activity, is titratable, and is modulated by autophosphorylation.
Mol Cell Biol. 31 (8): 1719-33.
Shrivastav, M. et al. (2009) DNA-PKcs and ATM co-regulate DNA double-strand break repair.
DNA Repair (Amst). 8: 920-9.
Pettitt, A.R. et al. (2001) p53 dysfunction in B-cell chronic lymphocytic leukemia: inactivation of ATM as an alternative to TP53 mutation.
Blood. 98 (3): 814-22.
Henriksson, G. et al. (2004) Enhanced DNA-dependent protein kinase activity in Sjögren's syndrome B cells.
Rheumatology (Oxford). 43: 1109-15.