ATG7 antibody

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Rabbit anti ATG7

ATG7 antibody recognizes the ubiquitin-like modifier-activating enzyme ATG7, also known as ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, autophagy-related protein 7, hAGP7 or ubiquitin activating enzyme E1-like protein.

ATG7 Antibody with Control Lysate

ATG7 antibody recognizes the ubiquitin-like modifier-activating enzyme ATG7, also known as ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, autophagy-related protein 7, hAGP7 or ubiquitin activating enzyme E1-like protein.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00230 WB 100 µl
VPA00230KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human ATG7 antibody recognizes ubiquitin-like modifier-activating enzyme ATG7, also known as ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, autophagy-related protein 7, hAGP7 and ubiquitin activating enzyme E1-like protein.

ATG7 was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes (provided by RefSeq, Jan 2009).

Rabbit anti Human ATG7 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATG7 detects a band of approximately 78 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O95352 Related reagents
Entrez Gene
ATG7 Related reagents
GO Terms
GO:0004839 ubiquitin activating enzyme activity
GO:0005737 cytoplasm
GO:0006497 protein lipidation
GO:0006914 autophagy
GO:0006944 cellular membrane fusion
GO:0015031 protein transport
GO:0019778 APG12 activating enzyme activity
GO:0031401 positive regulation of protein modification process
GO:0042803 protein homodimerization activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATG7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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