ATG5 antibody

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Rabbit anti ATG5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2160 P WB 50 µg
Rabbit anri ATG5 antibody recognises human autophagy related 5 homolog (ATG5).

ATG5 is an essential protein involved in the autophagy process and conjugates to ATG12.

ATG5 may play an important role in apoptosis where it is expressed late in the apoptopic process downstream of caspase activity.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Zebrafish Expected from Sequence
Bovine Expected from Sequence
Pig Expected from Sequence
Rat Expected from Sequence
Mouse Expected from Sequence
Fish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to ATG5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Synthetic peptide sequence corresponding to a region between amino acids 1-50 of human ATG5 - conjugated to KLH.
Approx. Protein Concentrations
Total protein concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9H1Y0 Related reagents
Entrez Gene
ATG5 Related reagents
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005776 autophagic vacuole
GO:0032480 negative regulation of type I interferon production
GO:0034045 pre-autophagosomal structure membrane
GO:0043687 post-translational protein modification
Regulatory
For research purposes only

Applications of ATG5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 5ug/ml
Western Blotting 0.5 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human kidney

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P