ATG4B antibody
Rabbit anti ATG4B antibody recognizes human Cysteine protease ATG4B, also known as AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1 or Autophagy-related protein 4 homolog B. ATG4B is a 393 amino acid ~45 kDa cytoplasmic cysteine protease. ATG4B is expressed mainly in skeletal muscle tissue but also in brain, heart, liver and pancreas (Mariño et al. 2003).
ATG4 is involved in cytoplasm to vacuole transport and is involved in the progress of autophagy (Scherz-Shouval et al. 2007).
ATG4 is involved in cytoplasm to vacuole transport and is involved in the progress of autophagy (Scherz-Shouval et al. 2007).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Zebrafish Expected from Sequence Xenopus Expected from Sequence Mouse Expected from Sequence Rat Expected from Sequence Chicken Expected from Sequence Fish Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to ATG4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography on Protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Synthetic peptide corresponding to a portion of the amino acid sequence between 300-450 of human ATG4 – KLH conjugated.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of ATG4B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 10 ug/ml | ||
| Western Blotting | 1 ug/ml | 3 ug/ml |
- 1 This product requires antigen retrieval using heat treatment. Citrate buffer, pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human heart
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Histar Detection System | STAR3000A | C P | 50 Tests | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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