ATG4B antibody

100% Secure


Rabbit anti ATG4B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2098 P* WB 50 µg
Rabbit anti ATG4B antibody recognizes human Cysteine protease ATG4B, also known as AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1 or Autophagy-related protein 4 homolog B. ATG4B is a 393 amino acid ~45 kDa cytoplasmic cysteine protease. ATG4B is expressed mainly in skeletal muscle tissue but also in brain, heart, liver and pancreas (Mariño et al. 2003).

ATG4 is involved in cytoplasm to vacuole transport and is involved in the progress of autophagy (Scherz-Shouval et al. 2007).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Zebrafish Expected from Sequence
Xenopus Expected from Sequence
Mouse Expected from Sequence
Rat Expected from Sequence
Chicken Expected from Sequence
Fish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to ATG4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Synthetic peptide corresponding to a portion of the amino acid sequence between 300-450 of human ATG4 – KLH conjugated.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y4P1 Related reagents
Entrez Gene
ATG4B Related reagents
Regulatory
For research purposes only

Applications of ATG4B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10 ug/ml
Western Blotting 1 ug/ml 3 ug/ml
  1. 1 This product requires antigen retrieval using heat treatment. Citrate buffer, pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P