ATG4A antibody

Rabbit anti Human ATG4A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ATG4A

Product Code Applications Pack Size List Price Your Price Qty
AHP2220
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Rabbit anti Human ATG4A antibody recognises human ATG4A (Autophagy-related protein 4 homolog A), otherwise known as APG4A/Cysteine protease ATG4A, a cytosolic cysteine protease and member of the peptidase C54 family, predominantly expressed in skeletal muscle and brain, which plays a central role in the cleavage of the C-Terminal region of the target proteins GABARAPL2, MAP1LC3 or GABARAP during autophagy.

Rabbit anti Human ATG4A antibody (AHP2220) has been shown to be suitable for use in immunoprecipitation using HEK-293T cell lysates.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ATG4A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 100-114 of human ATG4A. The corresponding sequence differs by 2 amino acids in mouse and rat.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 5 10ug
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
APG4A
Cysteine Protease ATG4A
RRID
AB_10846926
UniProt
Q8WYN0
Entrez Gene
ATG4A
View more products with ATG4 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products