ATG16 antibody
Rabbit anti ATG16
ATG16 antibody recognizes Autophagy-related protein 16-1, also known as APG16-like 1. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00086 | WB | 100 µl |
Rabbit anti Human ATG16 antibody recognizes human autophagy-related protein 16 (ATG16). ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions.
Rabbit anti Human ATG16 antibody detects autophagy-related protein 16 as a band of approximately 65 kDa in a range of human cell line lysates.
Rabbit anti Human ATG16 antibody detects autophagy-related protein 16 as a band of approximately 65 kDa in a range of human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti ATG16 detects a band of approximately 65 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to an 18 aa sequence from near the amino terminus of human ATG16.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
More Information
- UniProt
- Q676U5 Related reagents
- Entrez Gene
- ATG16L1 Related reagents
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005515 protein binding
- GO:0005776 autophagic vacuole
- GO:0015031 protein transport
- GO:0034045 pre-autophagosomal structure membrane
- GO:0051260 protein homooligomerization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ATG16 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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