ATG16 antibody

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Anti ATG16 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ATG16 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/50,000, STAR208P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to ATG16 (molecular weight 65 kDa)

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Rabbit anti ATG16

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ATG16

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VPA00086 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human ATG16 antibody recognizes human autophagy-related protein 16 (ATG16). ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions.

Rabbit anti Human ATG16 antibody detects autophagy-related protein 16 as a band of approximately 65 kDa in a range of human cell line lysates.

Target Species: Human
Western Blotting: Rabbit anti ATG16 detects a band of approximately 65 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Synthetic peptide corresponding to an 18 aa sequence from near the amino terminus of human ATG16.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

Synonyms: APG16-Like 1
UniProt: Q676U5
Entrez Gene: ATG16L1
GO Terms: GO:0000045 autophagic vacuole assembly; GO:0005515 protein binding; GO:0005776 autophagic vacuole; GO:0015031 protein transport; GO:0034045 pre-autophagosomal structure membrane; GO:0051260 protein homooligomerization