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ATG16 antibody

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Western Blot Validated
Anti ATG16 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ATG16 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/50,000, STAR208P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to ATG16 (molecular weight 65 kDa)

Rabbit anti ATG16

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00086
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human ATG16 antibody recognizes human autophagy-related protein 16 (ATG16). ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions.

Rabbit anti Human ATG16 antibody detects autophagy-related protein 16 as a band of approximately 65 kDa in a range of human cell line lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti ATG16 detects a band of approximately 65 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an 18 aa sequence from near the amino terminus of human ATG16.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q676U5
Entrez Gene
ATG16L1
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0005776 autophagic vacuole
GO:0015031 protein transport
GO:0034045 pre-autophagosomal structure membrane
GO:0051260 protein homooligomerization
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATG16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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