ATG16 antibody

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Rabbit anti ATG16 (Internal Region)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1894 C WB 0.1 mg
Rabbit anti ATG16 (Internal Region) antibody recognizes human Autophagy-related protein 16 (ATG16).

ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-Terminal domain, it has been suggested that these may form a platform for further protein-protein interactions.

Multiple isoforms of ATG16 exist.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ATG16 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an 18 aa sequence from near the centre of human ATG16.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q676U5 Related reagents
Entrez Gene
ATG16L1 Related reagents
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0005776 autophagic vacuole
GO:0015031 protein transport
GO:0034045 pre-autophagosomal structure membrane
GO:0051260 protein homooligomerization
Regulatory
For research purposes only

Applications of ATG16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 0.25 0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1894 detects a band of approximately 75 kDa in HeLa cell lysate.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P