ATG16 antibody

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Anti ATG16 (Internal Region) Antibody thumbnail image 1

Anti ATG16 (Internal Region) Antibody thumbnail image 2

Anti ATG16 (Internal Region) Antibody gallery image 1 — Western blot analysis of whole cell lysate from HeLa adenocarcinoma cells probed with Rabbit anti Human ATG16 (**AHP1894**) at 0.25(A) and 0.5(B) ug/ml.

Anti ATG16 (Internal Region) Antibody gallery image 2 — Immunocytochemical staining of HeLa cells with Rabbit anri Human ATG16 (**AHP1894**).

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Rabbit anti ATG16 (Internal Region)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: ATG16
Region: (INTERNAL REGION)

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Product Code Applications Pack Size List Price Your Price Qty

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AHP1894 Datasheet Datasheet SDS SDS	C WB	0.1 mg		Log in	Get Quote
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Rabbit anti ATG16 (Internal Region) antibody recognizes human Autophagy-related protein 16 (ATG16).

ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-Terminal domain, it has been suggested that these may form a platform for further protein-protein interactions.

Multiple isoforms of ATG16 exist.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human ATG16 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
Immunogen: Synthetic peptide corresponding to an 18 aa sequence from near the centre of human ATG16.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Western Blotting		0.25	0.5ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1894 detects a band of approximately 75 kDa in HeLa cell lysate.

Description	Product Code	Applications	Pack Size	Your Price
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

Synonyms: APG16-LIKE 1
RRID: AB_2059217
UniProt: Q676U5
Entrez Gene: ATG16L1
GO Terms: GO:0000045 autophagic vacuole assembly; GO:0005515 protein binding; GO:0005776 autophagic vacuole; GO:0015031 protein transport; GO:0034045 pre-autophagosomal structure membrane; GO:0051260 protein homooligomerization