ATG12 antibody

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Anti ATG12 (Internal Region) Antibody thumbnail image 1

Anti ATG12 (Internal Region) Antibody thumbnail image 2

Anti ATG12 (Internal Region) Antibody gallery image 1 — Western blot analysis of mouse heart lysate probed with Rabbit anti Human ATG12 (**AHP1891**) im the absence (A) or presence (B) of blocking peptide.

Anti ATG12 (Internal Region) Antibody gallery image 2 — Immunohistochemical staining of human brain tissue with Rabbit anti Human ATG12 (**AHP1891**).

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Rabbit anti ATG12 (Internal Region)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: ATG12
Region: (INTERNAL REGION)

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Product Code Applications Pack Size List Price Your Price Qty

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AHP1891 Datasheet Datasheet SDS SDS	P * WB	0.1 mg		Log in	Get Quote
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Rabbit anti ATG12 (Internal Region) antibody recognizes human Autophagy-related protein 12 (ATG12).

ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.

There are at least two isoforms of ATG12.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human ATG12 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
Immunogen: Synthetic peptide corresponding to a 15 aa sequence from near the centre of human ATG12.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹		2.5ug/ml
Western Blotting		1ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human brain
Western Blotting: AHP1891 detects a band of approximately 40 kDa in mouse heart tissue lysate.

Description	Product Code	Applications	Pack Size	Your Price
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

Synonyms: APG12-LIKE
RRID: AB_1898210
UniProt: O94817; Q9CQY1; Q2TBJ5
Entrez Gene: ATG12; Atg12; Atg12
GO Terms: GO:0000045 autophagic vacuole assembly; GO:0005515 protein binding; GO:0032480 negative regulation of type I interferon production; GO:0034045 pre-autophagosomal structure membrane; GO:0005624 membrane fraction; GO:0005625 soluble fraction; GO:0043234 protein complex; GO:0005829 cytosol