ATG101 antibody

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Rabbit anti ATG101

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2243 C E WB 0.1 mg
Rabbit anti ATG101 antibody recognises human autophagy-related protein 101 (ATG101), a 218 amino acid ~30 kDa protein belonging to the ATG101 family involved in autophagosome formation.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to Human ATG101 were raised by repeated immunisation of Rabbits with highly purified antigen Purified IgG prepared from whole serum by chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to 16 amino acid sequence from near the centre of human ATG101
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9BSB4 Related reagents
Q6AY69 Related reagents
Q9D8Z6 Related reagents
Entrez Gene
RGD1359310 Related reagents
C12orf44 Related reagents
9430023L20Rik Related reagents
GO Terms
GO:0000407 pre-autophagosomal structure
GO:0000045 autophagic vacuole assembly
GO:0032403 protein complex binding
Regulatory
For research purposes only

Applications of ATG101 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 5ug/ml
Western Blotting 1-2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue
Mouse Liver
Western Blotting
AHP2243 detects a band of approximately 29 kDa in Human liver tissue lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*