ATG1 antibody

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Rabbit anti ATG1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2214 P WB 50 µg
Rabbit anti Human ATG1 antibody recognises Human Serine/threonine-protein kinase ULK1, also known as Autophagy-related protein 1 homolog, ATG1 or Unc-51-like kinase 1. ATG1 is a 1050 amino acid ~115 kDa, ubiquitously expressed cytoplasmic protein belonging to the serine/threonine protein kinase family.

ATG1 is an autophagy related protein which is required for the formation of autophagosomes. ATG1 is the the target of the TOR kinase signalling pathway regulating autophagy and forms a complex with ATG13 and RB1CC1.

ATG1 is strongly expressed in a wide range of adult tissues including heart, lung, kidney, brain, liver, pancreatic tissue and skeletal muscle.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ULK1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
A synthetic peptide corresponding to an amino acid sequence within the region 320-370 of Human ULK1.
Approx. Protein Concentrations
Total protein concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O75385 Related reagents
Entrez Gene
ULK1 Related reagents
GO Terms
GO:0000407 pre-autophagosomal structure
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0008104 protein localization
GO:0005524 ATP binding
GO:0005776 autophagic vacuole
GO:0006914 autophagy
GO:0010506 regulation of autophagy
GO:0032403 protein complex binding
GO:0070969 ULK1-ATG13-FIP200 complex
Regulatory
For research purposes only

Applications of ATG1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 10ug/ml
Western Blotting 1 3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human kidney

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P