ATF6 antibody

100% Secure


Goat anti ATF6

ATF6 (aa437-449) antibody recognizes cyclic AMP-dependent transcription factor ATF-6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha, activating transcription factor 6 alpha or ATF6-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.

ATF6 Antibody with Control Lysate

ATF6 (aa437-449) antibody recognizes cyclic AMP-dependent transcription factor ATF-6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha, activating transcription factor 6 alpha or ATF6-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00107 WB 100 µl
VPA00107KT WB 2 Westerns
With Lysate 400µg
Goat anti Human ATF6 antibody recognizes activating transcription factor 6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha.

Encoded by the ATF6 gene, this protein is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, ATF6 is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. ATF6 has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in ATF6 with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. ATF6 is also thought to be a potential therapeutic target for cystic fibrosis (provided by RefSeq, Aug 2011).

Goat anti Human ATF6 antibody detects a band of 84 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATF6 detects a band of approximately 84 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-NSYRYDHSVSNDK, from the internal region of the protein sequence
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P18850 Related reagents
Entrez Gene
ATF6 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0006457 protein folding
GO:0016021 integral to membrane
GO:0005635 nuclear envelope
GO:0005654 nucleoplasm
GO:0005789 endoplasmic reticulum membrane
GO:0006990 positive regulation of gene-specific transcription involved in unfolded protein response
GO:0043565 sequence-specific DNA binding
GO:0046983 protein dimerization activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATF6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted