ATF6 antibody

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Goat anti ATF6

ATF6 (aa437-449) antibody recognizes cyclic AMP-dependent transcription factor ATF-6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha, activating transcription factor 6 alpha or ATF6-alpha. The antibody has been extensively validated for western blotting using whole cell lysates.

ATF6 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00107	WB	100 µl
VPA00107KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Goat anti Human ATF6 antibody recognizes activating transcription factor 6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha.

Encoded by the ATF6 gene, this protein is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, ATF6 is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. ATF6 has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in ATF6 with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. ATF6 is also thought to be a potential therapeutic target for cystic fibrosis (provided by RefSeq, Aug 2011).

Goat anti Human ATF6 antibody detects a band of 84 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti ATF6 detects a band of approximately 84 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Preparation: 20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution: TRIS buffered saline
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C-NSYRYDHSVSNDK, from the internal region of the protein sequence
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P18850 Related reagents
Entrez Gene: ATF6 Related reagents
GO Terms: GO:0000139 Golgi membrane; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0003713 transcription coactivator activity; GO:0006457 protein folding; GO:0016021 integral to membrane; GO:0005635 nuclear envelope; GO:0005654 nucleoplasm; GO:0005789 endoplasmic reticulum membrane; GO:0006990 positive regulation of gene-specific transcription involved in unfolded protein response; GO:0043565 sequence-specific DNA binding; GO:0046983 protein dimerization activity
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of ATF6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

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