ATF6 antibody

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Goat anti ATF6

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00107
Datasheet
WB
MSDS
100 µl

Goat anti Human ATF6 antibody recognizes activating transcription factor 6 alpha, also known as cAMP-dependent transcription factor ATF-6 alpha.

Encoded by the ATF6 gene, this protein is a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, ATF6 is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. ATF6 has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in ATF6 with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. ATF6 is also thought to be a potential therapeutic target for cystic fibrosis (provided by RefSeq, Aug 2011).

Goat anti Human ATF6 antibody detects a band of 84 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATF6 detects a band of approximately 84 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-NSYRYDHSVSNDK, from the internal region of the protein sequence

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P18850
Entrez Gene
ATF6
GO Terms
GO:0000139 Golgi membrane
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0006457 protein folding
GO:0016021 integral to membrane
GO:0005635 nuclear envelope
GO:0005654 nucleoplasm
GO:0005789 endoplasmic reticulum membrane
GO:0006990 positive regulation of gene-specific transcription involved in unfolded protein response
GO:0043565 sequence-specific DNA binding
GO:0046983 protein dimerization activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATF6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader