ATF2 (pThr52) antibody

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Rabbit anti ATF2 (pThr52)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2272 WB* 0.1 ml
Rabbit anti ATF2 (pThr52) antibody recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 52.

The ATF2 gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Several studies implicate abnormal activation of ATF-2 in growth and progression of mammalian skin tumors.

ATF2 may mediate oncogenesis caused by mutant Ras protein and regulate maintenance of the aggressive cancer phenotype of some types of epithelial cells. ATF2 has also been shown to be phosphorylated by ATM at Serine 490 and 498 in humans. The serine phosphorylated forms play a role in mediating DNA repair and in response to DNA damage induced by ionizing radiation (IR).

ATF2 exhibits histone acetyltransferase (HAT) activity and has been reported to specifically acetylate histones H2B and H4 in vitro. In concert with CUL3 and RBX1, ATF2 promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. This protein can also elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

In response to stress stimuli, ATF2 activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype. Inhibiting ATF2 impedes melanoma development and elicits tumor suppressorfunction. To act as a tumor suppressor, ATF2 must localize at the mitochondria and phosphorylation at Thr52 by PKCε regulates this translocation.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Primate Expected from Sequence
Rat Expected from Sequence
Xenopus Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ATF2 (pThr52) was raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr52 (pThr52) region of human ATF2

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P15336 Related reagents
Entrez Gene
ATF2 Related reagents
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0008270 zinc ion binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043565 sequence-specific DNA binding
GO:0045087 innate immune response
GO:0046983 protein dimerization activity
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of ATF2 (pThr52) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/250
  1. 1 For the detection of phosphoproteins, phosphatase inhibitors should always be added freshly to the lysis buffer. Milk or other casein-based blocking solutions are generally not recommended for blocking; as casein is itself a phosphoprotein its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Human ATF2 detects a band of approximately 72 kDa in UACC903 melanoma cell line lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB