Rabbit anti ATF2 (pThr51/pThr69) antibody
recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 51 or threonine 69. ATF2 is a member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. ATF2 plays a role in the regulation of genes involved in cell differentiation, growth and the response to stress and DNA damage.
ATF2 binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 1997
). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro
(Kawasaki et al. 2000
ATF2 is phosphorylated at threonine 69 and threonine 71 by JNK1, JNK2 and p38 MAPK, enhancing the transcriptional activity of ATF2 (Yamasaki et al. 2009
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ATF2 (pThr51/pThr69) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 51/threonine 69 of human ATF2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
RNA polymerase II transcription factor activity
transcription coactivator activity
Toll signaling pathway
zinc ion binding
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
sequence-specific DNA binding
innate immune response
protein dimerization activity
regulation of transcription factor activity
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ATF2 (pThr51/pThr69) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Shuman, J.D. et al. (1997) ATF-2 and C/EBPalpha can form a heterodimeric DNA binding complex in vitro. Functional implications for transcriptional regulation.
J Biol Chem. 272 (19): 12793-800.
Kawasaki, H. et al. (2000) ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation.
Nature. 405 (6783): 195-200.
Yamasaki, T. et al. (2009) Phosphorylation of Activation Transcription Factor-2 at Serine 121 by Protein Kinase C Controls c-Jun-mediated Activation of Transcription.
J Biol Chem. 284 (13): 8567-81.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra