ATF2 (pThr51/pThr69) antibody
Rabbit anti ATF2 (pThr51/pThr69) antibody recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 51 or threonine 69. ATF2 is a member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. ATF2 plays a role in the regulation of genes involved in cell differentiation, growth and the response to stress and DNA damage.
ATF2 binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 1997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).
ATF2 is phosphorylated at threonine 69 and threonine 71 by JNK1, JNK2 and p38 MAPK, enhancing the transcriptional activity of ATF2 (Yamasaki et al. 2009).
ATF2 binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 1997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).
ATF2 is phosphorylated at threonine 69 and threonine 71 by JNK1, JNK2 and p38 MAPK, enhancing the transcriptional activity of ATF2 (Yamasaki et al. 2009).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ATF2 (pThr51/pThr69) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 51/threonine 69 of human ATF2
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P15336 Related reagents
- Entrez Gene
- ATF2 Related reagents
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0008270 zinc ion binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043565 sequence-specific DNA binding
- GO:0045087 innate immune response
- GO:0046983 protein dimerization activity
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- Regulatory
- For research purposes only
Applications of ATF2 (pThr51/pThr69) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Shuman, J.D. et al. (1997) ATF-2 and C/EBPalpha can form a heterodimeric DNA binding complex in vitro. Functional implications for transcriptional regulation.
J Biol Chem. 272 (19): 12793-800. -
Kawasaki, H. et al. (2000) ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation.
Nature. 405 (6783): 195-200. -
Yamasaki, T. et al. (2009) Phosphorylation of Activation Transcription Factor-2 at Serine 121 by Protein Kinase C Controls c-Jun-mediated Activation of Transcription.
J Biol Chem. 284 (13): 8567-81.
