Rabbit anti ATF2 (pThr51/pThr69) antibody
recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 51 or threonine 69. ATF2 is a member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. ATF2 plays a role in the regulation of genes involved in cell differentiation, growth and the response to stress and DNA damage.
ATF2 binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 1997
). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro
(Kawasaki et al. 2000
ATF2 is phosphorylated at threonine 69 and threonine 71 by JNK1, JNK2 and p38 MAPK, enhancing the transcriptional activity of ATF2 (Yamasaki et al. 2009
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ATF2 (pThr51/pThr69) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 51/threonine 69 of human ATF2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
RNA polymerase II transcription factor activity
transcription coactivator activity
Toll signaling pathway
zinc ion binding
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
sequence-specific DNA binding
innate immune response
protein dimerization activity
regulation of transcription factor activity
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ATF2 (pThr51/pThr69) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Shuman, J.D. et al. (1997) ATF-2 and C/EBPalpha can form a heterodimeric DNA binding complex in vitro. Functional implications for transcriptional regulation.
J Biol Chem. 272 (19): 12793-800.
Kawasaki, H. et al. (2000) ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation.
Nature. 405 (6783): 195-200.
Yamasaki, T. et al. (2009) Phosphorylation of Activation Transcription Factor-2 at Serine 121 by Protein Kinase C Controls c-Jun-mediated Activation of Transcription.
J Biol Chem. 284 (13): 8567-81.
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