ATF2 antibody

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Goat anti ATF2

The ATF2 antibody recognizes cyclic AMP-dependent transcription factor ATF-2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, or cAMP responsive element binding protein 2.

ATF2 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size
VPA00027	WB	100 µl
VPA00027KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Goat anti Human ATF2 antibody recognizes human Cyclic AMP-dependent transcription factor ATF-2 (ATF2), also known as Activating transcription factor 2, HB16, CREB-2, Histone acetyltransferase ATF2 or cAMP response element-binding protein CRE-BP1. ATF2 is a 505 amino acid member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. Multiple isoforms of ATF2 are generated by alternative splicing, Goat anti Human ATF2 antibody was directed against the carboxy-terminal region (aa 492-505) and sequence suggests that the antibody may bind isoforms 1, 2, 4, 5, 6 and 8 with appropriate changes in recognized molecular weight.

Goat anti Human ATF2 antibody binds to epitopes within the C-terminal (CT) region of human ATF2 which binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 19997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).

Goat anti Human ATF2 antibody recognizes human ATF2 as bands of ~65 and 75kDa by western blotting under reducing conditions.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography

Preparation

20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography

Buffer Solution

TRIS buffered saline

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin

Immunogen

Peptide sequence C-IVMAPSSQSQPSGS from the C-terminal region of ATF2

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P15336 Related reagents
Entrez Gene: ATF2 Related reagents
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0003713 transcription coactivator activity; GO:0005654 nucleoplasm; GO:0008063 Toll signaling pathway; GO:0008270 zinc ion binding; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0043565 sequence-specific DNA binding; GO:0045087 innate immune response; GO:0046983 protein dimerization activity; GO:0051090 regulation of transcription factor activity; GO:0051403 stress-activated MAPK cascade
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of ATF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti ATF2 antibody detects bands of approximately 65 and 75 kDa in Jurkat cell lysates.
Western Blotting: Anti ATF2 antibody detects bands of approximately 65 and 75 kDa in Jurkat cell lysates.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB
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