ATF2 antibody

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Anti ATF2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ATF2 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 31 second exposure. Arrow points to ATF2 (molecular weight 65, 75 kDa)

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Goat anti ATF2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: ATF2

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VPA00027 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human ATF2 antibody recognizes human Cyclic AMP-dependent transcription factor ATF-2 (ATF2), also known as Activating transcription factor 2, HB16, CREB-2, Histone acetyltransferase ATF2 or cAMP response element-binding protein CRE-BP1. ATF2 is a 505 amino acid member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. Multiple isoforms of ATF2 are generated by alternative splicing, Goat anti Human ATF2 antibody was directed against the carboxy-terminal region (aa 492-505) and sequence suggests that the antibody may bind isoforms 1, 2, 4, 5, 6 and 8 with appropriate changes in recognized molecular weight.

Goat anti Human ATF2 antibody binds to epitopes within the C-terminal (CT) region of human ATF2 which binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 19997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).

Goat anti Human ATF2 antibody recognizes human ATF2 as bands of ~65 and 75kDa by western blotting under reducing conditions.

Target Species: Human
Western Blotting: Anti ATF2 antibody detects bands of approximately 65 and 75 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin.
Immunogen: Peptide sequence C-IVMAPSSQSQPSGS from the C-terminal region of ATF2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P15336
Entrez Gene: ATF2
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0003713 transcription coactivator activity; GO:0005654 nucleoplasm; GO:0008063 Toll signaling pathway; GO:0008270 zinc ion binding; GO:0034130 toll-like receptor 1 signaling pathway; View More GO Terms; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0043565 sequence-specific DNA binding; GO:0045087 innate immune response; GO:0046983 protein dimerization activity; GO:0051090 regulation of transcription factor activity; GO:0051403 stress-activated MAPK cascade