ATF2 antibody
Goat anti ATF2
The ATF2 antibody recognizes cyclic AMP-dependent transcription factor ATF-2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, or cAMP responsive element binding protein 2.
ATF2 Antibody with Control Lysate
The ATF2 antibody recognizes cyclic AMP-dependent transcription factor ATF-2, also known as activating transcription factor 2 splice variant ATF2-var2, cAMP response element-binding protein CRE-BP1, or cAMP responsive element binding protein 2.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00027 | WB | 100 µl | ||||
| VPA00027KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human ATF2 antibody recognizes human Cyclic AMP-dependent transcription factor ATF-2 (ATF2), also known as Activating transcription factor 2, HB16, CREB-2, Histone acetyltransferase ATF2 or cAMP response element-binding protein CRE-BP1. ATF2 is a 505 amino acid member of the ATF family of transcription factors containing single bZIP and C2H2-type zinc finger domains. Multiple isoforms of ATF2 are generated by alternative splicing, Goat anti Human ATF2 antibody was directed against the carboxy-terminal region (aa 492-505) and sequence suggests that the antibody may bind isoforms 1, 2, 4, 5, 6 and 8 with appropriate changes in recognized molecular weight.
Goat anti Human ATF2 antibody binds to epitopes within the C-terminal (CT) region of human ATF2 which binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 19997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).
Goat anti Human ATF2 antibody recognizes human ATF2 as bands of ~65 and 75kDa by western blotting under reducing conditions.
Goat anti Human ATF2 antibody binds to epitopes within the C-terminal (CT) region of human ATF2 which binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 19997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).
Goat anti Human ATF2 antibody recognizes human ATF2 as bands of ~65 and 75kDa by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ATF2 antibody detects bands of approximately 65 and 75 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-IVMAPSSQSQPSGS from the C-terminal region of ATF2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P15336 Related reagents
- Entrez Gene
- ATF2 Related reagents
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0008270 zinc ion binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043565 sequence-specific DNA binding
- GO:0045087 innate immune response
- GO:0046983 protein dimerization activity
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ATF2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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