Goat anti Human ATF2 antibody binds to epitopes within the C-terminal (CT) region of human ATF2 which binds to the cAMP responsive element (CRE), stimulating CRE- dependent transcription (Shuman et al. 19997). ATF2 also functions as a histone acetyltransferase (HAT), specifically acetylating histones 2B and 4 in vitro (Kawasaki et al. 2000).
Goat anti Human ATF2 antibody recognizes human ATF2 as bands of ~65 and 75kDa by western blotting under reducing conditions.
- Target Species
- Western Blotting
- Anti ATF2 antibody detects bands of approximately 65 and 75 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Peptide sequence C-IVMAPSSQSQPSGS from the C-terminal region of ATF2
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P15336 Related reagents
- Entrez Gene
- ATF2 Related reagents
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0008270 zinc ion binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043565 sequence-specific DNA binding
- GO:0045087 innate immune response
- GO:0046983 protein dimerization activity
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of ATF2 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||C E P WB||1 ml|
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