Asns antibody

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Rabbit anti Asns

ASNS antibody recognizes ASNS, also known as TS11 cell cycle control protein or glutamine-dependent asparagine synthetase.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00845 WB 100 µl
Rabbit anti Human ASNS antibody recognizes ASNS, also known as TS11 cell cycle control protein, asparagine synthetase [glutamine-hydrolyzing] and glutamine-dependent asparagine synthetase.

The protein encoded by ASNS is involved in the synthesis of asparagine. ASNS complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for ASNS (provided by RefSeq, May 2010).

Rabbit anti Human ASNS detectects a band of 64 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ASNS antibody recognizes a band of approximately 64 kDa in PC-12 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human ASNS
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P08243 Related reagents
Entrez Gene
ASNS Related reagents
GO Terms
GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0006529 asparagine biosynthetic process
GO:0006541 glutamine metabolic process
GO:0042149 cellular response to glucose starvation
GO:0043066 negative regulation of apoptosis
GO:0045931 positive regulation of mitotic cell cycle
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Asns antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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