ASK1 (pSer83) antibody

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Rabbit anti ASK1 (pSer83)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1242
Datasheet
E WB
MSDS
50 µg loader

Rabbit anti ASK1 (pSer83) antibody recognizes apoptosis signal-regulating kinase 1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5) and MAPK/ERK kinase kinase 5 (MEKK 5), when phosphorylated at serine 83. ASK1 is a serine/threonine kinase that plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at serine 83 to inhibit ASK1 induced apoptosis.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to ASK1 (pSER83) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Approx. Protein Concentrations
IgG concentration 2.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q99683 Related reagents
Entrez Gene
MAP3K5 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0006915 apoptosis
GO:0004709 MAP kinase kinase kinase activity
GO:0005524 ATP binding
GO:0007257 activation of JUN kinase activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0008656 caspase activator activity
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of ASK1 (pSer83) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5,000 1/10,000
Western Blotting 1/1,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1242 detects a band of approximately 155kDa in SW1353 cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

Further Reading

  1. Zhang, R. et al. (2005) Hsp90-Akt phosphorylates ASK1 and inhibits ASK1-mediated apoptosis.
    Oncogene. 24: 3954-3963.
  2. Hayakawa, T. et al. (2006) The ASK1-MAP kinase pathways in immune and stress responses.
    Microbes Infect. 8: 1098-1107.
  3. Takeda, K. et al. (2003) Roles of MAPKKK ASK1 in stress-induced cell death.
    Cell Struct. Funct. 28: 23-29.