ASK1 (pSer83) antibody

Rabbit anti ASK1 (pSer83)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ASK1 (pSer83)

Product Code Applications Pack Size List Price Your Price Qty
AHP1242
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 50 µg loader
List Price Your Price
loader

Rabbit anti ASK1 (pSer83) antibody recognizes apoptosis signal-regulating kinase 1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5) and MAPK/ERK kinase kinase 5 (MEKK 5), when phosphorylated at serine 83. ASK1 is a serine/threonine kinase that plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at serine 83 to inhibit ASK1 induced apoptosis.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to ASK1 (pSER83) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Approx. Protein Concentrations
IgG concentration 2.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5,000 1/10,000
Western Blotting 1/1,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1242 detects a band of approximately 155kDa in SW1353 cell lysates.

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Further Reading

  1. Zhang, R. et al. (2005) Hsp90-Akt phosphorylates ASK1 and inhibits ASK1-mediated apoptosis.
    Oncogene. 24: 3954-3963.
  2. Hayakawa, T. et al. (2006) The ASK1-MAP kinase pathways in immune and stress responses.
    Microbes Infect. 8: 1098-1107.
  3. Takeda, K. et al. (2003) Roles of MAPKKK ASK1 in stress-induced cell death.
    Cell Struct. Funct. 28: 23-29.

Synonyms
Apoptosis SIGNAL-REGULATING Kinase 1
RRID
AB_844577
UniProt
Q99683
Entrez Gene
MAP3K5
GO Terms
GO:0000287 magnesium ion binding
GO:0006915 apoptosis
GO:0004709 MAP kinase kinase kinase activity
GO:0005524 ATP binding
GO:0007257 activation of JUN kinase activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0008656 caspase activator activity
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms

AHP1242

100214

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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