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ASK1 antibody

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Anti Human ASK1 (C-Terminal) Antibody thumbnail image 1 Anti Human ASK1 (C-Terminal) Antibody thumbnail image 2
Anti Human ASK1 (C-Terminal) Antibody gallery image 1
Western Blot analysis of whole cell lysate from SW1353 chondrosarcoma cell line probed with Rabbit anti Human ASK1 (AHP1594).
Anti Human ASK1 (C-Terminal) Antibody gallery image 2
Immunocytochemical staining of A431 epithelial carcinoma cell line with Rabbit anti Human ASK1 (AHP1594)

Rabbit anti Human ASK1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1594
Datasheet
C WB
SDS
0.1 mg loader

Rabbit anti Human ASK1 antibody recognises human ASK1, also known as apoptosis signal-regulating kinase 1, MAP kinase kinase kinase or MAPKKK. ASK1 is a ~155 kDa protein belonging to the protein kinase superfamily.

ASK1 is a serine/threonine kinase and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.

ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human ASK1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human ASK1.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q99683
Entrez Gene
MAP3K5
GO Terms
GO:0000287 magnesium ion binding
GO:0006915 apoptosis
GO:0004709 MAP kinase kinase kinase activity
GO:0005524 ATP binding
GO:0007257 activation of JUN kinase activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0008656 caspase activator activity
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of ASK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 10ug/ml
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1594 detects a band of approximately 155kDa in SW1353 cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Ichijo H., et al (1997) Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.
    Science 275:90-4.
  2. Wang XS, et al (1996) Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase.
    J Biol Chem 271:31607-11.
  3. Tobiume, K. et al. (1997) Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1.
    Biochem Biophys Res Commun. 239 (3): 905-10.

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