Rabbit anti ARNT antibody recognizes aryl hydrocarbon receptor nuclear translocator (encoded by the gene ARNT), also known as hypoxia-inducible factor 1-beta (HIF1-beta) or bHLHe2. ARNT is a member of the bHLH PAS (basic helix-loop-helix Per-ARNT-Sim) family of transcription factors. ARNT forms complexes with either hypoxia-inducible factor 1-alpha (HIF-1 alpha) or aryl hydrocarbon receptor (AhR), ARNT is involved in the adaptive response to stress caused by dioxins and hypoxia.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ARNT was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human ARNT
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to hypoxia
positive regulation of endothelial cell proliferation
sequence-specific DNA binding transcription factor activity
transcription coactivator activity
signal transducer activity
positive regulation vascular endothelial growth factor production
positive regulation of hormone biosynthetic process
aryl hydrocarbon receptor binding
transcription regulator activity
positive regulation of vascular endothelial growth factor receptor signaling pathway
mRNA transcription from RNA polymerase II promoter
positive regulation of gene-specific transcription
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of erythrocyte differentiation
positive regulation of glycolysis
protein heterodimerization activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ARNT antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti ARNT antibody detects a band of approximately 87 kDa in cell lysates under reducing conditions. Rabbit anti ARNT antibody also appears to detect a modified form of ARNT at a higher molecular weight.
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Secondary Antibodies Available
Useful Reagents Available
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