ARNT antibody

Rabbit anti ARNT

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ARNT

Product Code Applications Pack Size List Price Your Price Qty
AHP2436
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl loader
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loader
Search for Batch Specific Datasheets

Rabbit anti ARNT antibody recognizes aryl hydrocarbon receptor nuclear translocator (encoded by the gene ARNT), also known as hypoxia-inducible factor 1-beta (HIF1-beta) or bHLHe2. ARNT is a member of the bHLH PAS (basic helix-loop-helix Per-ARNT-Sim) family of transcription factors. ARNT forms complexes with either hypoxia-inducible factor 1-alpha (HIF-1 alpha) or aryl hydrocarbon receptor (AhR), ARNT is involved in the adaptive response to stress caused by dioxins and hypoxia.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ARNT was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human ARNT
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti ARNT antibody detects a band of approximately 87 kDa in cell lysates under reducing conditions. Rabbit anti ARNT antibody also appears to detect a modified form of ARNT at a higher molecular weight.

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UniProt
P27540
Entrez Gene
ARNT
GO Terms
GO:0001666 response to hypoxia
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0046886 positive regulation of hormone biosynthetic process
GO:0017162 aryl hydrocarbon receptor binding
GO:0030528 transcription regulator activity
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043193 positive regulation of gene-specific transcription
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045821 positive regulation of glycolysis
GO:0046982 protein heterodimerization activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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