Arhgdib antibody

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Rabbit anti Arhgdib

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00730 WB 100 µl
Rabbit anti Human ARHGDIB antibody recognizes the Rho GDP-dissociation inhibitor 2, also known as Rho GDI 2, rho GDP-dissociation inhibitor 2, or rho-GDI beta.

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (Scherle et al. 1993).

Rabbit anti Human ARHGDIB antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti ARHGDIB detects a band of approximately 27 kDa in THP-1 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the middle region of human ARHGDIB
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P52566 Related reagents
Entrez Gene
ARHGDIB Related reagents
GO Terms
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0005094 Rho GDP-dissociation inhibitor activity
GO:0005096 GTPase activator activity
GO:0005856 cytoskeleton
GO:0006928 cellular component movement
GO:0006955 immune response
GO:0007162 negative regulation of cell adhesion
GO:0007266 Rho protein signal transduction
GO:0007275 multicellular organismal development
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0051056 regulation of small GTPase mediated signal transduction
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Arhgdib antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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