ARFGAP3 antibody
Goat anti ARFGAP3 recognises ARFGAP3 (ADP - ribosylation factor GTPase activating protein 3), originally described as ARFGAP1.
ARFGAP3 is thought to promote hydrolysis of ARF1 bound GTP, which is required for the dissociation of coat proteins from Golgi derived membranes and vesicles.
ARFGAP3 is widely expressed, being found at high levels in endocrine glands and testis (Zheng et al. 2000).
ARFGAP3 is thought to promote hydrolysis of ARF1 bound GTP, which is required for the dissociation of coat proteins from Golgi derived membranes and vesicles.
ARFGAP3 is widely expressed, being found at high levels in endocrine glands and testis (Zheng et al. 2000).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to ARFGAP3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide NGVVTSIQDRYGS derived from C terminus of ARFGAP3 protein.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NP61
- Entrez Gene
- ARFGAP3
- GO Terms
- GO:0000139 Golgi membrane
- GO:0016192 vesicle-mediated transport
- GO:0005829 cytosol
- GO:0006886 intracellular protein transport
- GO:0008060 ARF GTPase activator activity
- GO:0008270 zinc ion binding
- GO:0008565 protein transporter activity
- GO:0009306 protein secretion
- GO:0032312 regulation of ARF GTPase activity
- Regulatory
- For research purposes only
Applications of ARFGAP3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1ug/ml | 3ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP697 detects a band of approximately 55-60kDa in mouse brain cell lysates. A minimum incubation time of 1 hour is recommended is recommended with this antibody.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for ARFGAP3 antibody
-
Liu, X. et al. (2001) Functional characterization of novel human ARFGAP3.
FEBS Lett. 490 (1-2): 79-83. -
Zhang, C. et al. (2000) Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family.
Genomics. 63 (3): 400-8.
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