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ARFGAP3 antibody

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Anti Human ARFGAP3 Antibody thumbnail image 1 Anti Human ARFGAP3 Antibody thumbnail image 2
Anti Human ARFGAP3 Antibody gallery image 1
Western blot analysis of ARFGAP3 in mouse brain extract probed with Goat anti Human ARFGAP3 (AHP697)
Anti Human ARFGAP3 Antibody gallery image 2
Western blotting analysis of mouse brain extracts (35μg total protein in RIPA buffer) probed with Goat anti Human ARFGAP3 antibody (AHP697) at 2.0μgml-1. Primary incubated for 1 hour. Signal was detected by western blot using chemiluminescence

Goat anti Human ARFGAP3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP697
Datasheet
WB
SDS
0.1 mg

Goat anti ARFGAP3 recognises ARFGAP3 (ADP - ribosylation factor GTPase activating protein 3), originally described as ARFGAP1.

ARFGAP3 is thought to promote hydrolysis of ARF1 bound GTP, which is required for the dissociation of coat proteins from Golgi derived membranes and vesicles.

ARFGAP3 is widely expressed, being found at high levels in endocrine glands and testis (Zheng et al. 2000).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to ARFGAP3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Synthetic peptide NGVVTSIQDRYGS derived from C terminus of ARFGAP3 protein.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9NP61
Entrez Gene
ARFGAP3
GO Terms
GO:0000139 Golgi membrane
GO:0016192 vesicle-mediated transport
GO:0005829 cytosol
GO:0006886 intracellular protein transport
GO:0008060 ARF GTPase activator activity
GO:0008270 zinc ion binding
GO:0008565 protein transporter activity
GO:0009306 protein secretion
GO:0032312 regulation of ARF GTPase activity
Regulatory
For research purposes only

Applications of ARFGAP3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1ug/ml 3ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP697 detects a band of approximately 55-60kDa in mouse brain cell lysates. A minimum incubation time of 1 hour is recommended is recommended with this antibody.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for ARFGAP3 antibody

  1. Liu, X. et al. (2001) Functional characterization of novel human ARFGAP3.
    FEBS Lett. 490 (1-2): 79-83.
  2. Zhang, C. et al. (2000) Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family.
    Genomics. 63 (3): 400-8.
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