Apolipoprotein B antibody

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Sheep anti Human Apolipoprotein B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP214 ID 1 ml
Sheep anti Human apolipoprotein B antibody recognizes human apolipoprotein B, one of many components of VLDL, LDL and IDL particles responsible for carrying water insoluble lipids throughout the body.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Guinea Pig
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Apolipoprotein B were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from serum by ion exchange chromatography.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.01%Benzamidine
0.1%EACA
1mMEDTA

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04114 Related reagents
Entrez Gene
APOB Related reagents
Regulatory
For research purposes only

Applications of Apolipoprotein B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Apolipoprotein B antibody

  1. Hazell, L.J. et al. (1996) Presence of hypochlorite-modified proteins in human atherosclerotic lesions.
    J Clin Invest. 97: 1535-44.
  2. Ikejiri, A. et al. (2004) Effects of atorvastatin on triglyceride-rich lipoproteins, low-density lipoprotein subclass, and C-reactive protein in hemodialysis patients.
    Metabolism. 53: 1113-7.
  3. Dixon, J.L. et al. (2002) Apolipoprotein B is synthesized in selected human non-hepatic cell lines but not processed into mature lipoprotein.
    J Histochem Cytochem. 50: 629-40.
  4. Du, X. et al. (1998) Identification of two regions in apolipoprotein B100 that are exposed on the cytosolic side of the endoplasmic reticulum membrane.
    J Cell Biol. 141: 585-99.